{"product_id":"angiogenesis-methods-and-protocols-9781071620618","title":"Angiogenesis: Methods and Protocols","description":"\u003cp\u003e\u003c\/p\u003e\u003cblockquote\u003eThis book provides protocols for exploring transcriptional phenotyping, transcript analysis, cell biology, pathology, and physiological and developmental angiogenesis models. It is written for the Methods in Molecular Biology series and is an authoritative and up-to-date resource for researchers in the field. \u003c\/blockquote\u003e\u003cp\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e: Paperback \/ softback\u003cbr\u003e\u003cstrong\u003eLength\u003c\/strong\u003e: 429 pages\u003cbr\u003e\u003cstrong\u003ePublication date\u003c\/strong\u003e: 02 February 2023\u003cbr\u003e\u003cstrong\u003ePublisher\u003c\/strong\u003e: Springer-Verlag New York Inc.\u003cbr\u003e\u003c\/p\u003e \u003cp\u003e\u003cbr\u003eThis comprehensive book expands upon the foundation of lymphatic protocols, offering valuable tools for interpreting large datasets generated by angiogenesis research. By employing a range of techniques, including protocols for transcriptional phenotyping, tissue-specific heterogeneity exploration, and database mining of existing datasets, researchers can delve deeper into understanding the complexities of angiogenesis. In addition, bioinformatic techniques are seamlessly integrated alongside protocols for transcript analysis, cell biology, pathology, and physiological and developmental angiogenesis models. Written for the esteemed Methods in Molecular Biology series, the chapters encompass introductions to their respective topics, comprehensive lists of necessary materials and reagents, detailed step-by-step laboratory protocols, and insightful tips on troubleshooting and avoiding common pitfalls. Authoritative and timely, Angiogenesis: Methods and Protocols serves as a valuable compendium of practical approaches to angiogenesis and endothelial cell biology research, empowering researchers to explore these fields with greater precision and effectiveness.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e\u003cbr\u003eAngiogenesis, the process by which new blood vessels form, plays a crucial role in various physiological and pathological processes, including wound healing, tumor growth, and cardiovascular diseases. Understanding the molecular mechanisms underlying angiogenesis is essential for developing effective therapies and interventions. In recent years, significant progress has been made in the field of angiogenesis research, leading to the generation of large datasets that require comprehensive analysis.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eTranscriptional Phenotyping:\u003c\/strong\u003e\u003cbr\u003eOne of the key techniques used in angiogenesis research is transcriptional phenotyping, which involves the analysis of gene expression patterns in different cell types and tissues. This approach allows researchers to identify specific transcription factors and signaling pathways that are involved in angiogenesis. Protocols for transcriptional phenotyping have been developed to explore tissue-specific heterogeneity, analyze gene expression data, and identify biomarkers that can be used to predict angiogenesis outcomes.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eBioinformatic Techniques:\u003c\/strong\u003e\u003cbr\u003eIn addition to transcriptional phenotyping, bioinformatic techniques are widely used in angiogenesis research to analyze large datasets generated by high-throughput technologies. These techniques include database mining, statistical analysis, and machine learning algorithms. Database mining involves the retrieval and analysis of existing datasets to identify patterns and relationships that can help researchers understand the mechanisms underlying angiogenesis. Statistical analysis involves the use of statistical models and hypothesis testing to identify significant differences in gene expression or other biological variables between different groups or conditions. Machine learning algorithms, such as neural networks and random forests, can be used to analyze complex data and make predictions about angiogenesis outcomes.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eCell Biology and Pathology:\u003c\/strong\u003e\u003cbr\u003eCell biology and pathology protocols are also essential for understanding angiogenesis. These protocols involve the study of cell proliferation, migration, and differentiation in different angiogenesis contexts. They also include techniques for analyzing cell morphology, immunohistochemistry, and Western blotting. Pathology protocols involve the study of angiogenesis in different pathological conditions, such as cancer and cardiovascular diseases.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePhysiological and Developmental Angiogenesis Models:\u003c\/strong\u003e\u003cbr\u003ePhysiological and developmental angiogenesis models are used to study the mechanisms underlying angiogenesis in different physiological contexts. These models include in vitro assays, in vivo experiments, and computational simulations. In vitro assays involve the culture of cells in vitro and the study of their angiogenic properties using a range of techniques, such as wound healing assays, tube formation assays, and microfluidic devices. In vivo experiments involve the study of angiogenesis in animal models, such as mice and zebrafish, and the use of imaging techniques, such as fluorescence microscopy and ultrasound imaging. Computational simulations involve the use of computer models to simulate the dynamics of angiogenesis and to predict the effects of different interventions or genetic mutations.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eConclusion:\u003c\/strong\u003e\u003cbr\u003eIn conclusion, this detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research. By employing a range of techniques, including transcriptional phenotyping, bioinformatic techniques, cell biology, pathology, and physiological and developmental angiogenesis models, researchers can delve deeper into understanding the complexities of angiogenesis and develop effective therapies and interventions for a wide range of diseases. The authors of this book are well-known experts in the field, and their expertise and experience are evident in the comprehensive and up-to-date nature of the content. Angiogenesis: Methods and Protocols is a valuable resource for researchers, students, and clinicians interested in angiogenesis and endothelial cell biology research.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWeight\u003c\/strong\u003e: 846g\u003cbr\u003e\u003cstrong\u003eDimension\u003c\/strong\u003e: 254 x 178 (mm)\u003cbr\u003e\u003cstrong\u003eISBN-13\u003c\/strong\u003e: 9781071620618\u003cbr\u003e \u003cstrong\u003eEdition number\u003c\/strong\u003e: 1st ed. 2022\u003c\/p\u003e","brand":"Shulph Ink","offers":[{"title":"Paperback \/ softback","offer_id":44302328791290,"sku":"9781071620618","price":149.93,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0522\/4297\/2845\/products\/noImage_1_fdab6b01-7cef-4442-9172-6c83751d44e6.jpg?v=1687925260","url":"https:\/\/shulphink.com\/products\/angiogenesis-methods-and-protocols-9781071620618","provider":"Shulph Ink","version":"1.0","type":"link"}