{"product_id":"nonalcoholic-steatohepatitis-methods-and-protocols-9781071621271","title":"Non-Alcoholic Steatohepatitis: Methods and Protocols","description":"\u003cp\u003e\u003c\/p\u003e\u003cblockquote\u003e\n\u003cbr\u003eThis volume provides a comprehensive collection of laboratory protocols used to analyze non-alcoholic steatohepatitis (NASH). The chapters cover topics such as histological diagnosis, in vivo NASH model generation, hepatocyte and Kupffer cell isolation, human pluripotent stem cell-derived liver organoids, single-cell and RNA-sequencing, and exosomes and exosomal miRNAs extraction from mesenchymal stem cells. Written in the Methods in Molecular Biology series format, the book is a valuable resource for researchers studying NASH. \u003c\/blockquote\u003e\u003cp\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e: Hardback\u003cbr\u003e\u003cstrong\u003eLength\u003c\/strong\u003e: 361 pages\u003cbr\u003e\u003cstrong\u003ePublication date\u003c\/strong\u003e: 26 February 2022\u003cbr\u003e\u003cstrong\u003ePublisher\u003c\/strong\u003e: Springer-Verlag New York Inc.\u003cbr\u003e\u003c\/p\u003e \u003cp\u003e\u003cbr\u003eThis comprehensive volume presents a diverse range of laboratory protocols employed by researchers to investigate various facets of non-alcoholic steatohepatitis (NASH). The chapters within this book delve into diverse topics, including methods for histological diagnosis of NASH, the rationale behind establishing an in vivo NASH model, protocols for isolating hepatocytes, Kupffer cells, bone marrow-derived macrophages, and adipocytes, techniques to generate human pluripotent stem cells-derived liver organoids, single-cell and RNA sequencing, and a detailed account of extracting exosomes and exosomal miRNAs from mesenchymal stem cells. Written in the renowned Methods in Molecular Biology series format, the chapters encompass introductions to their respective subjects, comprehensive lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cbr\u003eThorough and practical, Non-Alcoholic Steatohepatitis: Methods and Protocols serves as a valuable resource for both novice and experienced investigators engaged in studying NASH. Moreover, it serves as an indispensable reference for basic and clinical researchers, as well as students, in the field of NASH.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWeight\u003c\/strong\u003e: 910g\u003cbr\u003e\u003cstrong\u003eDimension\u003c\/strong\u003e: 254 x 178 (mm)\u003cbr\u003e\u003cstrong\u003eISBN-13\u003c\/strong\u003e: 9781071621271\u003cbr\u003e \u003cstrong\u003eEdition number\u003c\/strong\u003e: 1st ed. 2022\u003c\/p\u003e","brand":"Shulph Ink","offers":[{"title":"Hardback","offer_id":44103159349498,"sku":"9781071621271","price":124.94,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0522\/4297\/2845\/products\/noImage_1_e80ed4ef-f6c7-4c8f-b99c-11943cced132.jpg?v=1669650631","url":"https:\/\/shulphink.com\/products\/nonalcoholic-steatohepatitis-methods-and-protocols-9781071621271","provider":"Shulph Ink","version":"1.0","type":"link"}