Advanced Mass Spectrometry-based Analytical Separation Techniques for Probing the Polar Metabolome

The analysis of polar and charged metabolites in biological samples is a challenging task in metabolomics. Novel mass spectrometry-based analytical tools have been developed to enable sensitive and efficient profiling of these metabolites. This book provides a comprehensive overview of these recent technological developments, including the use of chemical labelling strategies, RPLC-MS, HILIC-MS, ion-pair LC-MS, nano-LC-MS, capillary electrophoresis–mass spectrometry, ion-mobility MS, and NMR spectroscopy. Sample preparation is also emphasized, with recent solid-phase extraction and solid-phase micro-extraction methods discussed. Analytical techniques for chiral metabolic profiling are also considered.

Format: Hardback
Length: 346 pages
Publication date: 21 July 2021
Publisher: Royal Society of Chemistry

The analysis of polar and charged metabolites in biological samples presents a significant challenge in the realm of metabolomics. In recent years, novel mass spectrometry-based analytical tools have emerged to facilitate the sensitive and efficient profiling of polar ionogenic metabolites across various biological matrices. This comprehensive book aims to provide a thorough overview of these recent technological advancements.

The book delves into various topics, including the utilization of chemical labeling strategies for the analysis of polar metabolites through reversed-phase liquid chromatography-mass spectrometry (RPLC-MS). It also highlights the latest methodological developments in RPLC-MS, hydrophilic interaction liquid chromatography (HILIC)-MS, and ion-pair LC-MS approaches. Special attention is given to advancements in nano-LC-MS and capillary electrophoresis-mass spectrometry methods, which are particularly useful for profiling polar metabolites in small volume biological samples. Furthermore, the book explores the utility of ion-mobility MS and NMR spectroscopy in metabolomics.

Sample preparation plays a crucial role in the analytical workflow employed for metabolomics. Therefore, ample emphasis is placed on recent solid-phase extraction and solid-phase micro-extraction methods. Additionally, analytical techniques for chiral metabolic profiling are considered.

By discussing the state-of-the-art of proposed topics in one single book, this work aims to provide a comprehensive exploration of the polar metabolome. The use of relevant examples enhances the understanding and applicability of the information presented, making it valuable to an international audience of analytical and biomedical researchers in industry and academia.

Weight: 706g
Dimension: 164 x 240 x 28 (mm)
ISBN-13: 9781839161636