Base Editors: Methods and Protocols

This volume explores base editors (BEs), a CRISPR-based genome editing tool with diverse applications, including disease modeling, mutagenic mice generation, therapeutic strategies, and plant and bacterial use. Written for the Methods in Molecular Biology series, it provides step-by-step protocols, troubleshooting tips, and insights for researchers.

Format: Hardback
Length: 235 pages
Publication date: 03 January 2023
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into the realm of base editors (BEs), a remarkable CRISPR-based genome editing tool with an array of diverse applications. Providing a foundational understanding, the book explores the various types of BEs, their variants, and computational tools employed in their manipulation. It then proceeds to showcase experimental applications of BEs in disease modeling in mammalian cells and the generation of mutagenic mice. Therapeutic base editing strategies are also covered, encompassing delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles. Additionally, the book explores the use of BEs in plants and bacteria. Written in the esteemed Methods in Molecular Biology series, each chapter includes an introduction, a comprehensive list of necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding common pitfalls. Authored by esteemed experts in the field, Base Editors: Methods and Protocols serves as an invaluable guide for researchers seeking to harness the power of base editors to advance their studies across a wide range of disciplines.

Weight: 671g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071628782
Edition number: 1st ed. 2023