Biophysics of Molecular Chaperones: Function, Mechanisms and Client Protein Interactions

Molecular chaperones are essential for controlling protein quality in all living cells. This book discusses advanced experimental techniques for analyzing their function, with a focus on NMR methodology for atomic resolution studies. It is targeted at structural biology and biophysical chemistry practitioners and scientists interested in entering the field.

Format: Hardback
Length: 394 pages
Publication date: 01 November 2023
Publisher: Royal Society of Chemistry

Molecular chaperones play a vital role in ensuring the quality of proteins in all living cells. By gaining a deeper understanding of their atomic-level functions and interactions with client proteins, we can unravel the fundamental roles they play in biology. This book aims to bridge a gap in the literature by providing a comprehensive summary and discussion of advanced experimental techniques for analyzing molecular chaperones.

The majority of the contributions in this book focus on NMR methodology, which is widely recognized as the method of choice for conducting atomic resolution studies of molecular chaperone systems. NMR techniques enable researchers to observe the structures, dynamics, and interactions of chaperones and their client proteins in exquisite detail.

In addition to NMR, other notable biophysical approaches are considered to present the most current developments in exploring chaperone function and the transient and dynamic interactions with their client proteins. These approaches include X-ray crystallography, cryo-electron microscopy, and single-molecule fluorescence spectroscopy.

The target audience for this book includes both current practitioners of structural biology and biophysical chemistry as well as scientists who are interested in entering the field. It can serve as valuable supplementary reading for graduate students who are interested in gaining a deeper understanding of molecular chaperones and their roles in cellular processes.

In conclusion, this book offers a comprehensive and up-to-date overview of advanced biophysical methods for characterizing molecular mechanisms of molecular chaperones. By leveraging these techniques, researchers can gain a deeper understanding of the roles played by chaperones in protein quality control, cellular signaling, and disease development.

Weight: 778g
Dimension: 162 x 242 x 30 (mm)
ISBN-13: 9781839162824