Shulph Ink
Celiac Disease
Celiac Disease
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- More about Celiac Disease
Celiac Disease, Volume 179 in the Methods in Cell Biology series, provides novel and widely used techniques for celiac disease research, such as Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, and In vivo sensitization to gliadin by oral administration.
Format: Hardback
Length: 228 pages
Publication date: 21 August 2023
Publisher: Elsevier Science Publishing Co Inc
Celiac Disease, Volume 179 in the Methods in Cell Biology series, is a comprehensive resource that provides extensive protocols for conducting research on celiac disease. This volume covers a wide range of topics, including:
1. Pepsin trypsin-digested gliadin treatment of intestinal cells in vitro.
2. Ex vivo gliadin stimulation of intestinal cells.
3. Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers.
4. Intestinal permeability assessment using lactulose and mannitol.
5. In vivo sensitization to gliadin by oral administration.
6. Separation of epithelial and immune cells from intestinal biopsy samples.
7. Isolation and study of dendritic cells and macrophages from human intestinal samples.
8. Gobiotic mouse models to study gluten sensitivity.
9. In vitro differentiation of macrophages from peripheral blood cells of celiac patients.
10. Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge.
11. Isolation, cryopreservation, and thawing of peripheral blood mononuclear cells for downstream applications.
These protocols offer researchers valuable insights into the latest techniques used to study celiac disease, providing a foundation for further research and advancements in this field.
Weight: 638g
Dimension: 198 x 245 x 18 (mm)
ISBN-13: 9780443192029
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