Cell-Free Gene Expression: Methods and Protocols

Cell-free expression (CFE) is a powerful tool for next-generation synthetic biology applications, enabling researchers to perform metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. This detailed volume provides an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications, with step-by-step protocols and troubleshooting tips.

Format: Paperback / softback
Length: 435 pages
Publication date: 07 January 2023
Publisher: Springer-Verlag New York Inc.

This extensive volume delves into perspectives and methodologies utilizing cell-free expression (CFE) to facilitate next-generation synthetic biology applications. The first section focuses on tools for CFE systems, encompassing a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section presents a diverse range of applications for CFE systems, including metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Authored for the renowned Methods in Molecular Biology series, chapters feature introductions to their respective topics, comprehensive lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and helpful tips on troubleshooting and avoiding known pitfalls. Comprising a wealth of authoritative and practical information, Cell‐Free Gene Expression: Methods and Protocols serves as an invaluable guide for researchers seeking technical methods to current aspects of CFE and related applications.

Weight: 861g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071620007
Edition number: 1st ed. 2022