Chemical Tools in Microbiology 1

Clinical Microbiology,Volume 664 in the Methods in Enzymology series, highlights new advances in the field with interesting chapters on a variety of topics, such as Synthesis of chemical probes to study bacterial adenylating enzymes, Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux, Combining informatics with ABPP to identify serine hydrolyses in bacteria, A Ligand Selection Strategy Identifies Chemical Probes Targeting the Proteases of SARS-CoV-2, Activity-based probes for bacterial histidine kinases, Metabolomic approaches to enzyme function and pathway discovery, Identification of bile salt hydrolase activity in gut microbiota, and much more. Other chapters cover Multiplex fluorescence screening and identification using multiplex TMT, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via SPR, Site-Specific Siderocalin Binding to Ferric and Ferric-Free Enterobactin As Revealed by Mass Spectrometry, Proteomics of short-chain fatty acid probes in Salmonella, Development and application of highly sensitive labeling reagents for amino acids, and a variety of other timely topics.

Format: Hardback
Length: 306 pages
Publication date: 01 April 2022
Publisher: Elsevier Science & Technology

Clinical Microbiology,Volume 664 in the Methods in Enzymology series,is a comprehensive resource that showcases the latest advancements in the field. This new volume presents a diverse range of captivating chapters on various topics, such as the synthesis of chemical probes for studying bacterial adenylating enzymes, the development of fluoroquinolone-derived fluorescent probes for analyzing bacterial penetration and efflux, the integration of informatics with ABPP to identify serine hydrolyses in bacteria, a ligand selection strategy that identifies chemical probes targeting the proteases of SARS-CoV-2, activity-based probes for bacterial histidine kinases, metabolomic approaches to enzyme function and pathway discovery, the identification of bile salt hydrolase activity in gut microbiota, and much more.

In addition to these insightful chapters, the book also delves into multiplex fluorescence screening and identification using multiplex TMT, customized peptidoglycan surfaces for investigating innate immune recognition via SPR, site-specific siderocalin binding to ferric and ferric-free enterobactin as revealed by mass spectrometry, proteomics of short-chain fatty acid probes in Salmonella, the development and application of highly sensitive labeling reagents for amino acids, and a wide array of other timely and relevant topics.

This authoritative text serves as a valuable resource for researchers, students, and practitioners in clinical microbiology, enzymology, and related fields, providing them with the latest knowledge and tools to advance their understanding and research in this dynamic and rapidly evolving field.

Weight: 600g
Dimension: 167 x 238 x 21 (mm)
ISBN-13: 9780323912228