Chromatin Accessibility: Methods and Protocols

This book provides a comprehensive resource on profiling chromatin accessibility and its dynamics, covering bulk-cell methods, single-molecule and enzymatic approaches, imaging-based methods, and computational methods. It is written for the Methods in Molecular Biology series and is an authoritative and up-to-date reference for researchers studying chromatin accessibility in various biological contexts.

Format: Hardback
Length: 341 pages
Publication date: 21 February 2023
Publisher: Springer-Verlag New York Inc.

This extensive and comprehensive volume serves as a valuable resource for researchers seeking to explore the field of profiling chromatin accessibility and its dynamics. It begins with a section that delves into bulk-cell methods, which employ enzymatic cleavage of accessible DNA to obtain information about relative accessibility. These methods provide insights into the distribution of nucleosomes within the genome.

The book then proceeds to explore single-molecule and enzymatic approaches, which aim to map absolute occupancy/accessibility. These methods utilize advanced technologies such as DNA sequencing and fluorescence microscopy to provide precise measurements of chromatin structure and dynamics.

Furthermore, the book discusses emerging tools for mapping DNA accessibility and nucleosome positioning in single cells. These methods leverage advanced imaging techniques, such as super-resolution microscopy and single-molecule imaging, to visualize accessible chromatin in its nuclear context.

Additionally, the volume includes computational methods for processing and analyzing chromatin accessibility datasets. These methods assist researchers in extracting meaningful information from large and complex datasets, facilitating a deeper understanding of chromatin regulation and its role in various biological processes.

Written with the esteemed Methods in Molecular Biology series in mind, the chapters in this book feature introductions to their respective topics, comprehensive lists of necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and helpful tips on troubleshooting and avoiding common pitfalls.

Authoritative and timely, Chromatin Accessibility: Methods and Protocols serves as an indispensable reference for researchers engaged in studying diverse aspects of chromatin accessibility across various biological contexts. Chapter 6 is made available open access under a Creative Commons Attribution 4.0 International License via the link: link.springer.com.

Weight: 867g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071628980
Edition number: 1st ed. 2023