Chromatin: Methods and Protocols

This volume provides novel and well-established techniques to study chromatin biology, including DNA-associated proteins, DNA methylation, three-dimensional chromatin interactions, deep sequencing-based tools, and data analysis pipelines. Each chapter is written in the format of the highly successful Methods in Molecular Biology series and includes step-by-step protocols.

Format: Hardback
Length: 379 pages
Publication date: 02 February 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume offers groundbreaking techniques to advance the study of chromatin biology. It encompasses a wide range of methods, including both novel and well-established approaches, for analyzing DNA-associated proteins, DNA methylation, three-dimensional chromatin interactions, deep sequencing-based tools, and data analysis pipelines. Written in the format of the highly successful Methods in Molecular Biology series, each chapter provides an introduction to the topic, outlines the necessary materials and reagents, offers helpful tips on troubleshooting and potential pitfalls, and presents step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Chromatin: Methods and Protocols strives to deepen our understanding of how modified DNA and associated proteins influence the transcriptional output of the genome.

Chapter 12, titled "Genome-wide mapping and microscopy visualization of protein-DNA interactions by pA-DamID," is available open access under a Creative Commons Attribution 4.0 International License via the link: link.springer.com.

This chapter presents a novel method for visualizing protein-DNA interactions at the genome-wide level using a modified version of DamID (DNA-associated protein immunodetection). The pA-DamID technique involves the labeling of protein-bound DNA with a fluorescent marker, allowing for the identification and mapping of protein-DNA interactions in living cells. The chapter provides detailed protocols for performing pA-DamID experiments, including cell culture, sample preparation, immunofluorescence staining, and image analysis.

By utilizing these cutting-edge techniques, researchers can gain a deeper insight into the complex mechanisms of chromatin regulation and transcriptional control, which are essential for understanding gene expression and cellular processes. The comprehensive nature of this volume makes it an invaluable resource for both novice and experienced chromatin biologists, providing them with the tools and knowledge necessary to advance their research in this field.

Weight: 932g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071621394
Edition number: 1st ed. 2022