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Cilia: From Mechanisms to Disease-Part B
Cilia: From Mechanisms to Disease-Part B
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- More about Cilia: From Mechanisms to Disease-Part B
The book "Cilia: From Mechanisms to Disease Part B,Volume 176 of the Methods in Cell Biology series" covers various methods for studying cilia, including genetic mutants, transcriptional adaptation, fixation, immunolabeling, single-molecule imaging, and analysis in mouse cardiac lesion models and zebrafish brains. It also discusses clinical and molecular diagnosis in BBS, modeling ciliopathies, tracheal epithelial cilia motility and mucociliary function, high-speed super-resolution SPEED Microscopy, and siRNA delivery in retina explants.
Format: Hardback
Length: 270 pages
Publication date: 01 May 2023
Publisher: Elsevier Science Publishing Co Inc
Cilia: From Mechanisms to Disease Part B,Volume 176 of the Methods in Cell Biology series, presents an engaging collection of chapters on diverse topics. These include:
The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish.
Human LUHMES and NES cells as models for studying primary cilia in neurons.
Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations.
Single-molecule imaging in the primary cilium.
Methods to analyze primary cilia in mouse cardiac lesion models.
Methods to study motile ciliated cell types in a zebrafish brain mode.
And much more!
Other sections delve into Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome), Modeling ciliopathies in patient-derived primary cells, Analysis of motility and mucociliary function of tracheal epithelial cilia, High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium, Methods for siRNA delivery in retina explants, Methods to study primary cilia and autophagy in the brain, and more.
This comprehensive volume offers valuable insights into the study of cilia, their mechanisms, and their role in various diseases. It is a valuable resource for researchers and scholars in the field of cell biology and related disciplines.
Weight: 450g
Dimension: 152 x 229 (mm)
ISBN-13: 9780443185885
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