Shulph Ink
DNA-Encoded Chemical Libraries: Methods and Protocols
DNA-Encoded Chemical Libraries: Methods and Protocols
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- More about DNA-Encoded Chemical Libraries: Methods and Protocols
This book discusses protocols for synthesizing, screening, and analyzing DNA-encoded chemical libraries (DEL), including methods for solution phase library synthesis, DNA encoding of chemical structures, target protein design, screening, DEL qPCR, preparative PCR, and DNA sequence analysis. Written in the Methods in Molecular Biology series format, it includes step-by-step laboratory protocols, troubleshooting tips, and lists of necessary materials and reagents.
Format: Hardback
Length: 226 pages
Publication date: 10 September 2022
Publisher: Springer-Verlag New York Inc.
This comprehensive volume delves into the realm of protocols encompassing the synthesis, screening, and analysis of DNA-encoded chemical libraries (DEL). Within its chapters, readers will find detailed discussions on the various methods employed to practice DEL technology, including solution-phase library synthesis employing diverse chemistries, DNA encoding of chemical structures, the design, preparation, and analysis of target proteins and tool compounds, affinity selection-based screening of soluble protein targets, DEL qPCR, preparative PCR, and DNA sequence analysis, computational approaches utilized to analyze selections and identify compounds for resynthesis, and the comprehensive analysis of hit compounds. Written in the renowned Methods in Molecular Biology series format, each chapter offers an introduction to its respective topic, a comprehensive list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls.
Delivering cutting-edge and comprehensive coverage, DNA-Encoded Chemical Libraries: Methods and Protocols serves as a valuable resource for scientists engaged in DEL technology for drug discovery. By presenting a wealth of knowledge and practical insights, this book will undoubtedly contribute to the ongoing advancement of this important field.
Weight: 649g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071625446
Edition number: 1st ed. 2022
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