Endophytic Microbes: Isolation, Identification, and Bioactive Potentials

This book provides basic insight and protocols relating to endophytic microbes, divided into five major sections: isolation, bioactive metabolites production, endophytism, isolation and identification of endophytes, and bioactive potentials. It is authoritative and cutting-edge, aiming to provide comprehensive and accessible methods to undergraduate, graduate, and established scientists.

Format: Hardback
Length: 272 pages
Publication date: 26 November 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume offers essential insights and protocols related to endophytic microbes. The chapters are organized into five major sections, each dedicated to exploring key aspects such as basic isolation, bioactive metabolites production, endophytism, isolation and identification of endophytes, bioactive potentials, and screening of metabolites. Authored by renowned experts in the field, this book serves as a valuable resource for undergraduate students, graduate students, and established scientists seeking to delve into the world of endophytic microbes.

The first section, titled "Basic Isolation and Identification of Endophytic Microbes," provides a thorough introduction to the techniques and methodologies employed for isolating and identifying endophytic bacteria and fungi from various plant tissues. It covers topics such as sample preparation, culture media selection, isolation methods, and identification techniques, including molecular biology-based methods. This section serves as a foundation for further exploration of the bioactive properties and potential applications of endophytic microbes.

The second section, titled "Bioactive Metabolites Production by Endophytic Microbes," delves into the fascinating world of endophytic microorganisms and their ability to produce bioactive compounds. It explores the mechanisms underlying the production of these compounds, including genetic factors, environmental conditions, and interactions with host plants. The section highlights the importance of endophytic metabolites in various industries, such as agriculture, medicine, and pharmaceuticals, and discusses the potential applications of these compounds in the development of new drugs, fertilizers, and other bioproducts.

The third section, titled "Endophytism and Its Role in Plant Health," explores the relationship between endophytic microbes and plant health. It discusses the mechanisms by which endophytic microbes confer plant protection against biotic and abiotic stresses, such as pests, pathogens, drought, and heavy metals. The section also highlights the role of endophytic microbes in enhancing plant growth, productivity, and resistance to environmental stresses.

The fourth section, titled "Isolation and Identification of Endophytes," focuses on the isolation and identification of endophytic microbes from different plant species. It provides detailed protocols and techniques for isolating endophytic bacteria and fungi from various plant tissues, including roots, stems, leaves, and seeds. The section also discusses the identification of endophytic microbes using molecular biology-based methods, such as PCR and DNA sequencing.

The fifth section, titled "Bioactive Potentials and Screening of Metabolites," explores the potential bioactive properties of endophytic microbes and their metabolites. It discusses the screening methods employed to identify and isolate novel bioactive compounds from endophytic microbes, including high-throughput screening techniques such as liquid chromatography-mass spectrometry (LC-MS) and bioassays. The section also highlights the importance of studying the chemical composition and biological activities of endophytic metabolites in order to understand their potential applications in various fields.

In conclusion, Endophytic Microbes: Isolation, Identification, and Bioactive Potentials is an authoritative and cutting-edge resource that provides comprehensive and accessible methods to undergraduate, graduate, and established scientists seeking to explore the world of endophytic microbes. With its extensive coverage of topics, from basic isolation to bioactive metabolites production, endophytism, isolation and identification of endophytes, bioactive potentials, and screening of metabolites, this book serves as a valuable tool for advancing our understanding of these fascinating microorganisms and their potential applications in various industries.

Weight: 743g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071628263
Edition number: 1st ed. 2023