Epiblast Stem Cells: Methods and Protocols

This book provides up-to-date protocols for using epiblast stem cells to answer critical questions in mammalian development. It covers mouse primed pluripotent stem cells, characterization and study toolkits, and engineered gastrulation models. Written for the Method in Molecular Biology series, it includes step-by-step protocols, troubleshooting tips, and an ideal guide to overcome reproducibility problems.

Format: Paperback / softback
Length: 303 pages
Publication date: 30 April 2023
Publisher: Springer-Verlag New York Inc.

This comprehensive book is meticulously designed to provide the most up-to-date protocols in utilizing epiblast stem cells (EpiSCs) to address vital questions related to mammalian development. It begins by delving into the diverse spectrum of mouse primed pluripotent stem cells, paving the way for subsequent chapters that explore comprehensive toolkits for characterizing and studying these primed stem cells. Moreover, the volume extends its reach by delving into the engineering of gastrulation models using primed stem cells. Authored by renowned experts in the field, each chapter in this book includes an insightful introduction that provides a foundational understanding of the respective topic. Additionally, a comprehensive list of the necessary materials and reagents is provided, along with detailed, readily reproducible laboratory protocols that facilitate seamless execution of experiments. Furthermore, practical tips on troubleshooting and avoiding known pitfalls are included to enhance the reader's experience and ensure successful outcomes.

Epiblast Stem Cells: Methods and Protocols stands as an authoritative and practical guide, serving as an invaluable resource for researchers seeking to overcome the reproducibility problem by accessing a wealth of detailed state-of-the-art protocols. These protocols enable comprehensive analyses, allowing the reader to choose the appropriate cell type to model the epiblast. By presenting these protocols in a user-friendly format, this book empowers researchers to delve deeper into the complexities of epiblast stem cell research, contributing to our understanding of mammalian development and paving the way for future advancements in this field.

Weight: 603g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071622834
Edition number: 1st ed. 2022