Epigenome-Wide Association Studies: Methods and Protocols

This volume provides detailed protocols for epigenome-wide association studies, covering topics such as DNA methylation data, data processing, quality control, normalization, and statistical analysis. Written in the popular Methods in Molecular Biology series format, it includes step-by-step analysis pipelines and tips for troubleshooting.

Format: Hardback
Length: 229 pages
Publication date: 04 May 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into the intricate details of DNA methylation data, encompassing a wide range of features such as data processing pipelines, quality control measures, data normalization, and statistical methods for data analysis, confounding control, and batch effects. The chapters are organized into two sections: one focused on microarray-based methylation measures and the other on sequence-based measures. Written in the renowned Methods in Molecular Biology series format, each chapter begins with an introduction that provides a clear overview of its respective topic. It then lists the necessary methodologies and software packages, accompanied by step-by-step, readily reproducible analysis pipelines. Additionally, tips on troubleshooting and avoiding known pitfalls are provided to aid researchers in their endeavors.

Authoritative and cutting-edge, Epigenome-Wide Association Studies: Methods and Protocols aims to serve as a valuable practical guide for researchers seeking to advance their understanding in this field. By presenting a comprehensive collection of methods and protocols, this book empowers researchers to explore the epigenetic landscape and uncover the underlying mechanisms associated with various diseases and biological processes.

The significance of DNA methylation in gene regulation and its role in various biological processes cannot be overstated. Epigenome-Wide Association Studies (EWAS) have emerged as a powerful tool for identifying genetic variations that contribute to disease risk and developing personalized treatments. This volume provides a comprehensive framework for conducting EWAS, covering topics such as sample preparation, DNA methylation analysis, statistical modeling, and interpretation of results.

Furthermore, the book discusses the challenges and limitations associated with EWAS, including technical considerations, sample selection bias, and the need for robust quality control measures. It provides practical insights and recommendations to address these challenges and ensure the accuracy and reliability of EWAS findings.

In conclusion, Epigenome-Wide Association Studies: Methods and Protocols is a indispensable resource for researchers and practitioners interested in epigenetics and genomics. It offers a comprehensive and up-to-date overview of the latest methods and protocols in this field, enabling researchers to explore the epigenetic landscape and uncover valuable insights into human health and disease.

Weight: 656g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071619933
Edition number: 1st ed. 2022