Essential Genes and Genomes: Methods and Protocols

This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, focusing on CRISPR/Cas9-based and Tn-seq-based approaches. It is written for the highly successful Methods in Molecular Biology series and is an ideal guide for researchers attempting to strip genetics down to its fundamentals.

Format: Paperback / softback
Length: 434 pages
Publication date: 29 October 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive book offers cutting-edge insights into gene essentiality screenings across a diverse range of organisms. It delves into the identification of protein-coding genes and other genomic elements that are crucial for an organism's survival under specific conditions. The book begins with chapters focusing on CRISPR/Cas9-based approaches and Tn-seq-based approaches, which have revolutionized the field. However, later chapters also explore other techniques for exploring essential genes, such as bioinformatics methods. Written for the esteemed Methods in Molecular Biology series, each chapter includes an introduction to its respective topic, a comprehensive list of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and helpful tips on troubleshooting and avoiding common pitfalls. Thorough and authoritative, Essential Genes and Genomes: Methods and Protocols serves as an invaluable guide for researchers seeking to delve into the fundamentals of genetics.

Weight: 846g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071617229
Edition number: 1st ed. 2022