Estrogen Receptors: Methods and Protocols

This updated collection includes chapters on various techniques for studying estrogen receptors, mediated by ERα and ERβ, and the non-genomic membrane-bound G-protein coupled estrogen receptor (GPER/GPR30). The protocols cover standard methods like immunoblot and RT-qPCR, as well as newer technologies like PET imaging, reporter animal models, polysome fractionation, and more. Written for the Methods in Molecular Biology series, the chapters provide step-by-step, readily reproducible laboratory protocols, troubleshooting tips, and lists of necessary materials and reagents.

Format: Paperback / softback
Length: 427 pages
Publication date: 05 February 2023
Publisher: Springer-Verlag New York Inc.

This fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ) as well as the non-genomic membrane-bound G-protein coupled estrogen receptor (GPER/GPR30). The protocols range from standard methods and important laboratory workhorses such as immunoblot and RT-qPCR, to newer technologies such as PET imaging, reporter animal models, polysome fractionation, and many others, involving a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written for this highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Estrogen Receptors: Methods and Protocols, Second Edition serves as an ideal guide to researchers working to explore the vibrancy and excitement of the field of estrogen receptors and estrogen actions.

Estrogen receptors, key players in regulating cellular responses to estrogen, have garnered significant attention in recent years. The development of novel techniques and technologies has enabled researchers to study these receptors in greater detail, leading to a better understanding of their physiological and pathological roles. In this comprehensive collection, experts in the field have compiled chapters covering a wide range of techniques essential for advancing our knowledge of estrogen receptors.

The chapters begin with introductions that provide an overview of the respective topics, highlighting the importance of estrogen receptors in various biological processes. These introductions serve as a valuable resource for researchers who are new to the field. Following the introductions, the chapters detail the necessary materials and reagents required for each technique. The authors have carefully selected commonly used tools and provided detailed instructions on their usage, ensuring that researchers can execute the protocols with ease.

Step-by-step, readily reproducible laboratory protocols are provided for each technique. These protocols detail the experimental procedures, including sample preparation, incubation times, and analytical methods. The authors have taken care to provide clear and concise instructions, making it possible for researchers to follow the protocols accurately and obtain reliable results. Additionally, tips on troubleshooting and avoiding known pitfalls are included, ensuring that researchers can overcome potential challenges and maximize the efficiency of their experiments.

The protocols presented in this collection cover a diverse range of techniques, including immunoblotting, RT-qPCR, Western blotting, immunohistochemistry, and cell-based assays. These methods allow researchers to study the expression and localization of estrogen receptors, as well as their interactions with estrogen response elements and other proteins. By employing these techniques, researchers can gain insights into the mechanisms by which estrogen regulates gene expression, cell growth, and differentiation, as well as its effects on physiological and pathological processes.

Furthermore, the collection includes chapters on newer technologies such as PET imaging, reporter animal models, polysome fractionation, and mass spectrometry. These techniques provide powerful tools for studying estrogen receptor actions in vivo and in vitro, allowing researchers to gain a deeper understanding of the molecular mechanisms underlying estrogen-mediated responses.

Written for this highly successful Methods in Molecular Biology series, the chapters in this collection are authored by renowned experts in the field. Each chapter includes extensive references and a comprehensive list of relevant literature, providing researchers with a valuable resource for further exploration.

In conclusion, Estrogen Receptors: Methods and Protocols, Second Edition serves as an essential guide for researchers working to explore the complexities of estrogen receptors and their actions. The comprehensive collection of techniques and protocols presented in this book provides researchers with the tools they need to advance their understanding of estrogen receptors and their roles in various biological processes. Whether you are a novice or an experienced researcher, this book will be a valuable resource for your work in the field.

Weight: 832g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071619223
Edition number: 2nd ed. 2022