Food Allergens: Methods and Protocols

Food Allergens: Methods and Protocols is a comprehensive guide to studying food allergens, offering protocols for recombinant production, purification, IgE and T cell epitopes characterization, allergen structure description, cellular responses, and tolerance induction. Written for scientists at all stages, it provides practical tips and troubleshooting advice.

Format: Hardback
Length: 377 pages
Publication date: 23 September 2023
Publisher: Springer-Verlag New York Inc.

Food Allergens: Methods and Protocols is a comprehensive volume that offers a wide range of methods and protocols for studying food allergens. It covers various aspects of food allergy research, including recombinant production, purification procedures, IgE and T cell epitopes characterization, allergen structure description, cellular responses, and tolerance induction. Written for the highly successful Methods in Molecular Biology series, the book provides detailed introductions to each topic, lists of necessary materials and reagents, step-by-step and reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

The book is authored by experts in the field and serves as an ideal reference for scientists at all stages involved in the study of food allergy and allergenic components. It covers a wide range of techniques and animal models, making it a valuable resource for researchers in academia, industry, and government.

One of the key features of Food Allergens: Methods and Protocols is its emphasis on the characterization of food allergens. The book provides detailed protocols for recombinant production of allergens, purification procedures, and characterization of IgE and T cell epitopes. These protocols are essential for understanding the immunological properties of allergens and developing effective therapies for food allergy.

In addition to the characterization of allergens, the book also covers allergen structure description, cellular responses, and tolerance induction. It provides detailed protocols for studying the structure of allergens, their interactions with cells, and the mechanisms of tolerance induction. These protocols are important for understanding the pathogenesis of food allergy and developing new strategies for prevention and treatment.

Another important aspect of Food Allergens: Methods and Protocols is its practicality. The book includes step-by-step and reproducible laboratory protocols that can be easily followed by researchers. It also includes tips on troubleshooting and avoiding known pitfalls, which can help researchers save time and resources.

The book is divided into several sections, each covering a different aspect of food allergy research. The first section provides an overview of food allergy and allergenic components, including their history, prevalence, and clinical manifestations. The second section covers the characterization of food allergens, including recombinant production, purification procedures, and characterization of IgE and T cell epitopes. The third section covers allergen structure description, cellular responses, and tolerance induction, including studies on the role of allergens in immune responses, the development of allergic diseases, and the potential for tolerance induction.

The book also includes several case studies and examples of food allergy research. These case studies provide real-world examples of how food allergens can affect individuals and how researchers are working to develop new therapies and preventative measures.

In conclusion, Food Allergens: Methods and Protocols is a comprehensive volume that offers a wide range of methods and protocols for studying food allergens. It is written for scientists at all stages involved in the study of food allergy and allergenic components and provides detailed introductions to each topic, lists of necessary materials and reagents, step-by-step and reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Its emphasis on the characterization of food allergens, allergen structure description, cellular responses, and tolerance induction makes it an ideal reference for researchers in academia, industry, and government.

Dimension: 254 x 178 (mm)
ISBN-13: 9781071634523
Edition number: 1st ed. 2024