Biomedical photonics is a rapidly growing field that connects research in physics, optics, and electrical engineering with medical and biological applications. Tissue optical clearing is a technique that reduces the scattering of tissue and makes it more transparent, allowing for structural and functional analysis of tissues and cells at the sub-cellular level. This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods. It is addressed to biophysicist researchers, graduate students, postdocs of biomedical specialties, biomedical engineers, and physicians interested in the development and application of optical methods in medicine.

Format: Paperback / softback
Length: 658 pages
Publication date: 27 May 2024
Publisher: Taylor & Francis Ltd

Biomedical photonics is a rapidly evolving field that combines research in physics, optics, and electrical engineering with medical and biological applications. It offers a powerful tool for structural and functional analysis of tissues and cells, providing resolution and contrast that surpasses other methods. However, achieving high-resolution imaging at the sub-cellular level and translating these techniques for in vivo studies remain significant challenges. One approach to address these challenges is tissue optical clearing, which involves immersing tissues into optical clearing agents (OCAs) that reduce scattering and make tissue more transparent. This method has been successfully used in various applications, including in vitro and in vivo tissue optical clearing methods, and combination of tissue optical clearing with various optical imaging techniques for diagnosis.

Biomedical Photonics: A Comprehensive Introduction to Tissue Optical Clearing

Biomedical photonics is a rapidly evolving field that combines research in physics, optics, and electrical engineering with medical and biological applications. It offers a powerful tool for structural and functional analysis of tissues and cells, providing resolution and contrast that surpasses other methods. However, achieving high-resolution imaging at the sub-cellular level and translating these techniques for in vivo studies remain significant challenges. One approach to address these challenges is tissue optical clearing, which involves immersing tissues into optical clearing agents (OCAs) that reduce scattering and make tissue more transparent. This method has been successfully used in various applications, including in vitro and in vivo tissue optical clearing methods, and combination of tissue optical clearing with various optical imaging techniques for diagnosis.

Introduction to Tissue Optical Clearing

Tissue optical clearing is a technique that uses immersion of tissues into optical clearing agents (OCAs) to reduce scattering and make tissue more transparent. This method has been used in various applications, including in vitro and in vivo tissue optical clearing methods, and combination of tissue optical clearing with various optical imaging techniques for diagnosis. The basic principles of tissue optical clearing include the reduction of scattering, the enhancement of light absorption, and the improvement of tissue transparency. OCAs can be classified into two main types: hydrophilic and hydrophobic. Hydrophilic OCAs are water-soluble and can be easily absorbed by tissues, while hydrophobic OCAs are oil-soluble and can be used to clear tissues that are not water-soluble.

In Vitro Biological Applications of Tissue Optical Clearing

In vitro biological applications of tissue optical clearing include the study of cell morphology, cell viability, and cell function. Cell morphology can be studied using optical microscopy, while cell viability and function can be studied using various assays such as cell proliferation, cell migration, and cell death. Optical clearing agents can be used to enhance the visibility of cells and tissues, making it easier to observe and analyze their structure and function.

In Vivo Tissue Optical Clearing Methods

In vivo tissue optical clearing methods include the use of optical clearing agents (OCAs) that are injected into tissues or delivered through the bloodstream. OCAs can be used to clear tissues in a variety of organs, including the brain, heart, liver, and kidney. The use of OCAs in in vivo tissue optical clearing methods has been shown to improve the visualization of tissues and organs, making it easier to diagnose and treat diseases.

Combination of Tissue Optical Clearing and Various Optical Imaging Techniques for Diagnosis

Combination of tissue optical clearing and various optical imaging techniques for diagnosis can provide a comprehensive view of tissues and organs. Optical imaging techniques such as confocal microscopy, optical coherence tomography (OCT), and fluorescence microscopy can be used to study the structure and function of tissues and organs. Optical clearing agents can be used to enhance the visibility of these imaging techniques, making it easier to observe and analyze the images.

Conclusion

Biomedical photonics is a rapidly evolving field that offers a powerful tool for structural and functional analysis of tissues and cells. Tissue optical clearing is a technique that uses immersion of tissues into optical clearing agents (OCAs) to reduce scattering and make tissue more transparent. This method has been successfully used in various applications, including in vitro and in vivo tissue optical clearing methods, and combination of tissue optical clearing with various optical imaging techniques for diagnosis. The basic principles of tissue optical clearing include the reduction of scattering, the enhancement of light absorption, and the improvement of tissue transparency. OCAs can be classified into two main types: hydrophilic and hydrophobic. In vitro biological applications of tissue optical clearing include the study of cell morphology, cell viability, and cell function. In vivo tissue optical clearing methods include the use of optical clearing agents (OCAs) that are injected into tissues or delivered through the bloodstream. Combination of tissue optical clearing and various optical imaging techniques for diagnosis can provide a comprehensive view of tissues and organs.

Weight: 1590g
Dimension: 280 x 211 x 39 (mm)
ISBN-13: 9781032118697