Laboratory Practices in Microbiology

Laboratory Practices in Microbiology provides updated insights on methods of isolation and cultivation,morphology of microorganisms,the determination of biochemical activities,and physical and chemical effects on microorganisms. It is an essential book for students and researchers in microbiology, health sciences, food engineering, and technology, and medicine.

Format: Paperback / softback
Length: 292 pages
Publication date: 10 February 2021
Publisher: Elsevier Science & Technology

Laboratory Practices in Microbiology is a comprehensive and up-to-date resource that delves into various aspects of microbiology, encompassing isolation and cultivation techniques, morphology of microorganisms, biochemical activities, and physical and chemical effects on these organisms. The book is organized into several sections, each covering a specific topic.

The preparation of media and their sterilization is discussed in detail, providing insights into the essential steps involved in creating a conducive environment for microbial growth. The section also covers microorganisms in the environment, emphasizing their significance and distribution in different environments. Aseptic techniques are explored, emphasizing the importance of maintaining a sterile environment to prevent contamination and ensure the accuracy of experimental results.

Pure culture techniques are discussed, including methods such as streak plating, pour plating, and selective isolation, which allow for the growth of single microorganisms from a mixed sample. Preservation of cultures is crucial to maintain the viability and genetic integrity of microorganisms, and various methods, such as freezing, drying, and storing in culture media, are described.

Morphological characteristics of microorganisms are explored, using various staining techniques to highlight their structure, shape, and size. Wet-mount and hanging-drop techniques are introduced, enabling the observation and study of microorganisms under a microscope. Different staining techniques, such as Gram staining, acid-fast staining, and fluorescence microscopy, are described, allowing for the identification and characterization of different types of microorganisms.

Cultural and biochemical characteristics of bacteria are extensively covered, providing insights into the growth patterns, nutrient requirements, and metabolic activities of these organisms. Antimicrobial effects of agents on microorganisms are discussed, highlighting the importance of understanding the mechanisms of resistance and the development of effective antimicrobial therapies. Hand scrubbing in the removal of microorganisms is described, emphasizing the importance of proper hand hygiene to prevent the spread of infections.

Characteristics of fungi are explored, including their morphology, growth patterns, and reproductive mechanisms. Uses of bacteriophages in different applications, such as food safety, medicine, and biotechnology, are discussed, highlighting their potential as tools for genetic engineering and disease treatment.

In addition to these core sections, Laboratory Practices in Microbiology includes a wealth of information and applications. The book is designed to be accessible to students and researchers in microbiology, the health sciences, food engineering and technology, and medicine, as well as anyone working in a laboratory setting with microorganisms.

To enhance the understanding of the topics covered, the book includes numerous images and diagrams. These images help readers visualize the processes and techniques described, making the content more engaging and understandable.

In conclusion, Laboratory Practices in Microbiology is an essential book for students and researchers in microbiology, the health sciences, food engineering and technology, and medicine. It provides comprehensive and up-to-date information on various aspects of microbiology, including isolation and cultivation techniques, morphology, biochemical activities, and physical and chemical effects on microorganisms. The book's accessible format, extensive coverage, and inclusion of images and diagrams make it an invaluable resource for anyone seeking to advance their knowledge and understanding of microbiology.

Weight: 492g
Dimension: 154 x 228 x 22 (mm)
ISBN-13: 9780323910170