Leong's Manual of Diagnostic Biomarkers for Immunohistology

The fourth edition of this book provides practical guidance on the preparation, key uses, and pitfalls of antibodies for immunohistology in a unique A-Z format, with seventeen full-color infographics presenting information about specific biomarkers and their expression in tissues and prognosis. It has been expanded with new biomarkers and the removal of those that are no longer in common use, making it a concise and easy-to-use resource for busy readers.

Format: Hardback
Length: 532 pages
Publication date: 21 July 2022
Publisher: Cambridge University Press

Immunohistochemistry (IHC) is a crucial technique in pathology that plays a vital role in diagnostic, therapeutic, prognostic, and research applications. As the interest in biomarkers continues to surge, it is imperative for pathologists to have a comprehensive understanding of the correct application of IHC in their daily practice. This fully revised and updated fourth edition offers practical guidance on the preparation, key uses, and potential pitfalls of antibodies for immunohistology in a unique A-Z format. Seventeen full-color infographics provide detailed information about specific biomarkers, such as FOXL2, GATA3, and SOX10, highlighting their expression in tissues and their prognostic significance. Expanded with new biomarkers and the removal of those that are no longer in common use, this concise new edition follows a standardized structure for easy access by busy readers.

The preparation of samples for immunohistochemistry is a critical step in ensuring accurate and reliable results. This edition provides detailed instructions on tissue preparation, fixation, and processing, including the use of appropriate antibodies and stains. It also covers the importance of quality control measures, such as tissue microarray construction and validation, to ensure the reproducibility and reliability of IHC data.

Key uses of immunohistochemistry include the diagnosis of cancer, the identification of tumor subtypes, and the assessment of tumor staging and prognosis. IHC can also be used to study the expression of specific proteins or genes in tissues, which can provide valuable insights into the biological mechanisms underlying disease. This edition discusses the most commonly used antibodies and stains for different tumor types and highlights the advantages and limitations of each method.

One of the challenges of immunohistochemistry is the interpretation of results, which can be influenced by various factors such as antibody specificity, tissue heterogeneity, and technical variables. This edition provides guidance on interpreting IHC images, including the use of scoring systems and the interpretation of positive and negative controls. It also discusses the importance of correlating IHC results with other clinical and pathological data to obtain a comprehensive understanding of the patient's condition.

In addition to its diagnostic applications, immunohistochemistry has significant therapeutic implications. It can be used to guide treatment decisions by identifying specific biomarkers that can be targeted with therapeutic agents. IHC can also be used to monitor treatment response and predict disease recurrence. This edition discusses the use of immunohistochemistry in drug development and the potential applications of targeted therapy in cancer treatment.

Immunohistochemistry is a rapidly evolving field, with new biomarkers and techniques being developed continuously. This edition reflects the latest developments in the field, including the use of advanced imaging techniques, such as multiplex immunohistochemistry and immunofluorescence, and the integration of molecular data with IHC analysis. It also discusses the ethical considerations and privacy concerns associated with the use of patient samples for research purposes.

In conclusion, immunohistochemistry is a powerful tool in pathology that plays a critical role in diagnostic, therapeutic, prognostic, and research applications. This fully revised and updated fourth edition provides practical guidance on the preparation, key uses, and potential pitfalls of antibodies for immunohistology in a unique A-Z format. Seventeen full-color infographics provide detailed information about specific biomarkers, highlighting their expression in tissues and their prognostic significance. Expanded with new biomarkers and the removal of those that are no longer in common use, this concise new edition follows a standardized structure for easy access by busy readers. By mastering the techniques and interpreting the results of immunohistochemistry, pathologists can improve their diagnostic accuracy, guide treatment decisions, and contribute to advancing our understanding of disease.

Weight: 1310g
Dimension: 260 x 208 x 34 (mm)
ISBN-13: 9781108491570
Edition number: 4 Revised edition