Mapping Genetic Interactions

This volume provides methods for identifying synthetic lethal, genetic interactions in model systems, including human cancer cells, using various approaches such as RNA interference, CRISPR/Cas9, drug-gene interactions, and single cell sequencing. Written in the format of the highly successful Methods in Molecular Biology series, it aims to be a useful practical guide for researchers.

Format: Paperback / softback
Length: 361 pages
Publication date: 01 October 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into diverse methods for identifying synthetic lethal, genetic interactions across various model systems, encompassing human cancer cells. Organized into chapters, the book guides readers through genetic interactions in model organisms, RNA interference, CRISPR/Cas9-based genome editing technologies, drug-gene interactions, mapping chemical genetic interactions, synergistic drug-gene relations, single-cell sequencing, gene expression profiling, and novel genetic interactions. Written in the style of the renowned Methods in Molecular Biology series, each chapter begins with an introduction to the topic, provides a comprehensive list of necessary materials and reagents, includes helpful tips on troubleshooting and potential pitfalls, and presents step-by-step, readily reproducible protocols. Authored by esteemed experts in the field, Genetic Interaction Mapping serves as an authoritative and cutting-edge resource, aimed at providing valuable guidance to researchers seeking to advance their understanding in this dynamic field.

Weight: 721g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071617427
Edition number: 1st ed. 2021