MCB: CAR T Cells: Development, Characterization and Applications

MCB: CAR T Cells: Development, Characterization, and Applications, Volume 167 in the Methods in Cell Biology series, covers new advances in the field, such as high-efficiency genetic modification using CRISPR/Cpf1 system, biodistribution of chimeric antigen receptor-modified T cells, lentiviral vector generation, and -retroviral vector generation. It also includes flow cytometry detection and quantification of CAR T cells into solid tumors and evaluation of CAR-T cell cytotoxicity.

Format: Hardback
Length: 242 pages
Publication date: 15 February 2022
Publisher: Elsevier Science & Technology

MCB: CAR T Cells: Development, Characterization, and Applications, Volume 167 in the Methods in Cell Biology series, presents exciting new advancements in the field. This comprehensive volume offers insightful chapters on a diverse range of timely topics, such as the High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy, Determination of the Biodistribution of Chimeric Antigen Receptor-Modified T Cells against CD19 in NSG Mice, Generation of CAR-T cells using lentiviral vectors, Generation of CAR T-cells using ?-retroviral vector, Flow cytometry detection and quantification of CAR T cells into solid tumors, Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis, and much more.

The development of CAR T cells has revolutionized the field of immunotherapy, offering a promising treatment option for patients with various types of cancer. CAR T cells are engineered immune cells that are designed to target specific cancer cells by expressing a chimeric antigen receptor (CAR). The CAR consists of a protein fragment that recognizes a specific antigen on the surface of cancer cells, fused to signaling domains that activate T cells to kill the cancer cells.

One of the key challenges in CAR T-cell therapy is the efficient genetic modification of T cells to express the CAR. The CRISPR/Cpf1 system has emerged as a powerful tool for this purpose, allowing for precise and efficient editing of the T-cell genome. In this chapter, we will discuss the high-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy.

We will also explore the determination of the biodistribution of chimeric antigen receptor-modified T cells against CD19 in NSG mice. This study will provide valuable insights into the distribution of CAR T cells in the body and their potential efficacy in treating cancer.

Generation of CAR T cells using lentiviral vectors is another important aspect of CAR T-cell therapy. Lentiviral vectors are used to deliver the CAR gene into T cells, allowing them to express the CAR and target cancer cells. In this chapter, we will discuss the generation of CAR T cells using lentiviral vectors and their potential applications in treating cancer.

Generation of CAR T cells using ?-retroviral vector is another approach for CAR T-cell therapy. Retroviral vectors are used to deliver the CAR gene into T cells, allowing them to express the CAR and target cancer cells. In this chapter, we will discuss the generation of CAR T cells using ?-retroviral vector and their potential applications in treating cancer.

Flow cytometry detection and quantification of CAR T cells into solid tumors is an essential step in CAR T-cell therapy. This technique allows for the identification and quantification of CAR T cells in the tumor, which can be used to monitor the treatment response and adjust the treatment regimen as needed. In this chapter, we will discuss the flow.

Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis is an important tool for assessing the efficacy of CAR T-cell therapy. This technique allows for the real-time measurement of cell impedance, which is a measure of cell viability and cytotoxicity. In this chapter, we will discuss the evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis and its potential applications in CAR T-cell therapy.

In conclusion, CAR T cells have emerged as a promising treatment option for patients with various types of cancer. The development of CAR T cells has been driven by advances in genetic modification technology, such as CRISPR/Cpf1 system, and has been further enhanced by the use of lentiviral vectors, ?-retroviral vectors, and flow cytometry detection and quantification of CAR T cells into solid tumors. The evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis is an important tool for assessing the efficacy of CAR T-cell therapy and has the potential to improve patient outcomes. As the field of CAR T-cell therapy continues to evolve, we can expect to see further advancements in this area, leading to improved treatment options for patients with cancer.

Weight: 450g
Dimension: 229 x 152 (mm)
ISBN-13: 9780323855013