Methods in Stem Cell Biology - Part B

The Methods in Cancer Stem Cell Biology: Part B,Volume 171 book highlights advances in the field, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, and Identification and isolation of slow-cycling GSCs.

Format: Hardback
Length: 260 pages
Publication date: 01 August 2022
Publisher: Elsevier Science & Technology

Cancer stem cell biology is a rapidly evolving field that plays a crucial role in understanding and treating cancer. In this comprehensive volume, Part B, Volume 171 of the Methods in Cell Biology series, experts in the field present cutting-edge research on timely topics. The book explores various methods and techniques used to study cancer stem cells, which are believed to be the driving force behind cancer development and recurrence.

One of the highlights of this volume is the chapter on Orthotopic brain tumor models derived from glioblastoma stem-like cells. These models provide valuable insights into the biology of glioblastoma and offer potential opportunities for drug testing and personalized therapy. The chapter discusses the establishment of these models, their characterization, and the use of these models to study tumor growth, invasion, and drug response.

Another interesting chapter is on RNA sequencing in hematopoietic stem cells. Hematopoietic stem cells are the cells that give rise to all blood cells in the body, and their RNA sequencing provides valuable information about their genetic makeup and cellular processes. The chapter discusses the methods used for RNA sequencing, the analysis of RNA data, and the identification of novel biomarkers and therapeutic targets in hematopoietic stem cells.

Generation of inducible pluripotent stem cells from human dermal fibroblasts is another important topic covered in the volume. Inducible pluripotent stem cells are pluripotent cells that can be induced to differentiate into any cell type in the body. These cells have great potential for tissue engineering and regenerative medicine. The chapter discusses the methods used for generating inducible pluripotent stem cells, their characterization, and the use of these cells in drug discovery and tissue engineering.

In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering is another fascinating chapter. Dental pulp stem cells are cells that reside in the pulp of the tooth and have the potential to regenerate dental tissues. The chapter discusses the methods used for preparing dental pulp stem cell grafts, the characterization of these grafts, and their use in dental tissue regeneration.

Gene expression knockdown in chronic myeloid leukemia stem cells is another important topic covered in the volume. Chronic myeloid leukemia is a type of blood cancer that is characterized by the overproduction of abnormal blood cells. The chapter discusses the methods used for gene expression knockdown in chronic myeloid leukemia stem cells, the analysis of RNA data, and the identification of novel therapeutic targets in these cells.

Identification and isolation of slow-cycling GSCs is another critical topic discussed in the volume. Slow-cycling GSCs are a subpopulation of cancer stem cells that are resistant to chemotherapy and radiation therapy. The chapter discusses the methods used for identifying and isolating slow-cycling GSCs, the characterization of these cells, and the use of these cells in drug discovery and personalized therapy.

Assessment of CD133, EpCAM, and other markers of cancer stem cells is also covered in the volume. These markers are used to identify and isolate cancer stem cells from other cell types in the tumor. The chapter discusses the methods used for assessing these markers, the analysis of RNA data, and the identification of novel therapeutic targets in cancer stem cells.

In conclusion, Cancer Stem Cell Biology: Part B, Volume 171 of the Methods in Cell Biology series is a valuable resource for researchers and practitioners in the field of cancer stem cell biology. The book presents cutting-edge research on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, and Assessment of CD133, EpCAM, and other markers of cancer stem cells. The volume provides valuable insights into the biology of cancer stem cells and offers potential opportunities for drug discovery and personalized therapy.

Weight: 450g
Dimension: 229 x 152 (mm)
ISBN-13: 9780323900188