Monoamine Oxidase: Methods and Protocols

This detailed book examines the main methods to study mammalian monoamine oxidases (MAOs), ranging from cell biology to computational chemistry. It covers assays and techniques used to measure MAO enzymatic activity and perform inhibition studies, as well as computational methods applied to rational drug design approaches. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions, lists of materials and reagents, step-by-step protocols, and tips on troubleshooting.

Format: Hardback
Length: 254 pages
Publication date: 29 September 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive book delves into diverse methodologies employed to study mammalian monoamine oxidases (MAOs), encompassing cell biology to computational chemistry. It commences by presenting techniques for obtaining pure samples of MAO A and MAO B, followed by a discussion on assays and techniques utilized to measure MAO enzymatic activity and conduct inhibition studies. The volume further explores methods to address the cellular localization and function of MAOs, either in cell lines or in animal models, as well as computational methods applied to rational drug design approaches used to develop novel MAO inhibitors. Authored in the renowned Methods in Molecular Biology series format, chapters encompass introductions to their respective topics, comprehensive lists of necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls. Serving as an indispensable resource for scientists engaged in studying MAOs and other similar amine oxidase enzymes, Monoamine Oxidase: Methods and Protocols offers authoritative and practical insights into this field.

Weight: 700g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071626429
Edition number: 1st ed. 2023