Mutation Breeding in Coffee with Special Reference to Leaf Rust: Protocols

This book provides a comprehensive overview of current methodologies for improving resistance to leaf rust in coffee, a major disease affecting Arabica coffee production. It introduces mutation breeding as an efficient tool to increase the genetic diversity of Arabica coffee and presents practical methods for mutation induction and screening. The book is intended for students, researchers, plant pathologists, breeders, and growers interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications.

Format: Hardback
Length: 314 pages
Publication date: 20 August 2023
Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG

This comprehensive open-access book offers a thorough exploration of cutting-edge approaches for enhancing resistance to leaf rust in coffee, a vital global cash crop and beverage. Coffea arabica L. (Arabica), which accounts for approximately 60% of the world's coffee production, faces a significant challenge from coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix. Annually, this disease leads to losses exceeding $1 billion, significantly impacting Arabica coffee cultivation. Moreover, the narrow genetic base of Arabica coffee hinders genetic improvement efforts. This protocol book introduces mutation breeding as a powerful tool to enhance the genetic diversity of Arabica coffee, presenting practical methods for mutation induction and screening for CLR resistance. A brief review of current breeding strategies, challenges, and opportunities for Arabica coffee improvement is provided, along with a comprehensive survey of common coffee diseases, with a particular focus on CLR. Detailed protocols for mutation induction and screening are described, encompassing innovative techniques such as single-cell mutagenesis utilizing in vitro cell and tissue culture methods and genome-wide screening of induced mutations using genomics tools. Each protocol chapter includes an introduction and is supported by example results. Given the recent devastating impact of CLR epidemics on Arabica coffee production in Latin America, this book serves as a timely reference and guide for students, researchers, plant pathologists, breeders, growers, and end-users interested in developing novel coffee genotypes for genetic studies, breeding, and commercial applications.

Weight: 670g
Dimension: 235 x 155 (mm)
ISBN-13: 9783662672723
Edition number: 1st ed. 2023