Natural Killer (NK) Cells: Methods and Protocols

This detailed book provides a broad collection of methodologies for natural killer (NK) cell research, including isolation, differentiation, functional tests, and clinical applications. Written for the Methods in Molecular Biology series, it is an ideal guide for researchers looking to contribute to the further development of NK cell research.

Format: Paperback / softback
Length: 330 pages
Publication date: 30 March 2023
Publisher: Springer-Verlag New York Inc.

This comprehensive book offers a diverse range of methodologies for studying natural killer (NK) cells. It begins by providing various techniques for isolating and differentiating NK cells, followed by comprehensive functional tests, including cytotoxicity, viral infection, and metabolism assays. Additionally, the volume explores the clinical applications of NK cells, offering insights into their potential therapeutic roles in various diseases. Written in the esteemed Methods in Molecular Biology series, each chapter includes an introduction, a list of necessary materials and reagents, step-by-step laboratory protocols, and helpful tips on troubleshooting and avoiding common pitfalls. Authored by esteemed experts in the field, Natural Killer (NK) Cells: Methods and Protocols serves as an invaluable resource for researchers seeking to advance their understanding of NK cell research, both at the basic and clinical levels.

Isolation and Differentiation of NK Cells:
The book begins by discussing the methods for isolating and differentiating NK cells from peripheral blood and other tissues. These techniques include cell sorting, flow cytometry, and immunomagnetic separation. The authors provide detailed protocols for each step, including the use of specific antibodies and reagents to enrich and isolate NK cells.

Functional Tests:
The next section focuses on functional tests that can be performed on NK cells. These tests include cytotoxicity assays, which measure the ability of NK cells to kill target cells, and viral infection assays, which assess their antiviral response. Metabolism assays are also discussed, which help in understanding the metabolic pathways of NK cells and their role in immune regulation.

Clinical Applications:
The book explores the clinical applications of NK cells in various diseases, such as cancer, autoimmune disorders, and infectious diseases. The authors discuss the role of NK cells in immune surveillance and their potential use as therapeutic agents in these conditions. They also provide insights into the development of NK cell-based therapies and the challenges associated with their implementation.

Conclusion:
Natural Killer (NK) Cells: Methods and Protocols is a comprehensive and authoritative guide for researchers interested in studying NK cells. The book offers a diverse range of methodologies, from isolation and differentiation to functional tests and clinical applications. Written in a clear and concise manner, the book is accessible to researchers with varying levels of expertise. With its practical approach, it serves as an ideal resource for researchers looking to contribute to the further development of basic and clinical NK cell research.

Weight: 655g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071621622
Edition number: 1st ed. 2022