New Methods and Sensors for Membrane and Cell Volume Research

New Methods and Sensors for Membrane and Cell Volume Research,Volume 88 provides an overview of novel experimental approaches to study the cell membrane and the cytosol, with a focus on fluorescent and FRET-based sensors. It also covers other optical and non-optical methods.

Format: Hardback
Length: 426 pages
Publication date: 29 November 2021
Publisher: Elsevier Science & Technology

New Methods and Sensors for Membrane and Cell Volume Research,Volume 88 offers a comprehensive exploration of innovative experimental techniques aimed at studying the cell membrane and the underlying cytosol, which have recently garnered increased attention. While the book primarily focuses on fluorescent and FRET-based sensors, it also includes dedicated chapters on various optical methods, such as transmission microscopy variations, and non-optical approaches, including neutron scattering and mass spectrometry. This volume serves as a valuable resource, providing a rare review of experimental methodologies for investigating intracellular phase transitions, anion channels, membrane tension and dynamics, and other highly relevant topics of current interest within the field.

The cell membrane, a thin and dynamic barrier, plays a crucial role in regulating cellular processes. Researchers have developed various experimental techniques to study its structure, dynamics, and interactions with molecules and ions. One prominent approach is the use of fluorescent and FRET-based sensors, which allow for real-time monitoring of membrane properties and interactions. These sensors can detect changes in membrane potential, pH, lipid composition, and protein activity, providing valuable insights into cellular signaling and regulation.

In addition to fluorescent sensors, optical methods such as transmission microscopy have been used to study the cell membrane. Variations of transmission microscopy, including fluorescence microscopy, confocal microscopy, and electron microscopy, enable researchers to visualize the cell membrane in three dimensions and study its structure, organization, and dynamics at the nanoscale level. These methods can reveal the arrangement of lipids, proteins, and other membrane components, as well as their interactions and dynamics within the cell.

Neutron scattering and mass spectrometry are non-optical methods that have also been employed to study the cell membrane. Neutron scattering provides information about the structure and dynamics of the membrane, including its density, viscosity, and elasticity. It can also reveal the location and movement of proteins and other membrane components within the cell. Mass spectrometry, on the other hand, allows for the identification and quantification of membrane proteins and lipids, providing insights into their composition, function, and interactions.

Another important area of research in membrane and cell volume research is the study of intracellular phase transitions. These transitions involve the movement of molecules and ions between different compartments within the cell, and they play a crucial role in cellular functions such as signaling, metabolism, and transport. Researchers have developed experimental techniques to study these transitions, including fluorescence microscopy, electron microscopy, and X-ray diffraction. These methods can reveal the mechanisms and kinetics of phase transitions, as well as their effects on cellular function.

In addition to these areas of research, the book also includes chapters on anion channels, membrane tension and dynamics, and other topics of intense current interest. Anion channels are membrane proteins that facilitate the transport of ions across the cell membrane, and they play a critical role in cellular functions such as nerve signaling, muscle contraction, and kidney function. Researchers have developed experimental techniques to study anion channels, including patch clamp electrophysiology, fluorescence microscopy, and X-ray crystallography. These methods can reveal the structure, function, and regulation of anion channels, as well as their interactions with other membrane components and cellular signaling pathways.

Membrane tension and dynamics are also important aspects of cellular function, and they are influenced by a variety of factors, including lipid composition, protein activity, and environmental conditions. Researchers have developed experimental techniques to study membrane tension and dynamics, including pressure-sensitive dyes, fluorescence microscopy, and X-ray diffraction. These methods can reveal the mechanisms and kinetics of membrane tension and dynamics, as well as their effects on cellular function and structure.

In conclusion, New Methods and Sensors for Membrane and Cell Volume Research,Volume 88 provides a comprehensive and up-to-date overview of innovative experimental techniques for studying the cell membrane and the underlying cytosol. The book emphasizes the use of fluorescent and FRET-based sensors, as well as optical and non-optical methods, to explore a wide range of topics related to membrane and cell volume research. By leveraging these techniques, researchers can gain a deeper understanding of cellular processes and develop new therapies for diseases associated with membrane dysfunction.

Weight: 450g
Dimension: 229 x 152 (mm)
ISBN-13: 9780323911146