Next Generation Sequencing and Data Analysis

This textbook is a comprehensive guide to performing Next Generation Sequencing applications and their analytics,written for biomedical scientists and medical students. It provides step-by-step protocols and detailed explanations, enabling the end user to understand and interpret the data without prior bioinformatics or computer sciences knowledge.

Format: Paperback / softback
Length: 218 pages
Publication date: 05 May 2021
Publisher: Springer Nature Switzerland AG

This comprehensive textbook offers step-by-step protocols and in-depth explanations for RNA Sequencing, ChIP-Sequencing, and Epigenetic Sequencing applications. It serves as a valuable resource for readers seeking to gain expertise in Next Generation Sequencing data analysis, interpretation, and visualization. By providing a solid foundation in statistical background and software tools, the book empowers end users, including biomedical scientists and medical students, to effectively perform and comprehend diverse Next Generation Sequencing applications and their associated analytics, regardless of prior knowledge in bioinformatics or computer sciences.

The textbook is organized into several chapters, each dedicated to a specific Next Generation Sequencing application. It begins with an introduction to the principles and techniques of RNA Sequencing, covering topics such as sample preparation, library construction, and sequencing technology. The subsequent chapters delve into ChIP-Sequencing, providing detailed protocols for identifying protein-DNA interactions and analyzing chromatin structure. Epigenetic Sequencing is also explored, covering methods for studying DNA methylation and histone modifications.

Throughout the book, emphasis is placed on practical aspects and hands-on learning. Step-by-step protocols are provided, accompanied by clear illustrations and detailed explanations. The authors also offer valuable insights into data analysis, interpretation, and visualization, using popular software tools such as BEDTools, R, and Python. Additionally, the textbook includes case studies and real-world examples to reinforce the theoretical concepts and demonstrate the application of Next Generation Sequencing in biomedical research and clinical settings.

Written in a clear and accessible style, the textbook is suitable for both novice and experienced researchers in the field of genomics and molecular biology. It serves as a valuable resource for those seeking to advance their knowledge and skills in Next Generation Sequencing applications and their analytics. By providing a comprehensive and up-to-date coverage of these technologies, the textbook contributes to the growing field of genomics and its potential applications in healthcare and disease diagnosis.

Weight: 406g
Dimension: 170 x 239 x 17 (mm)
ISBN-13: 9783030624897
Edition number: 1st ed. 2021