Pericytes: Methods and Protocols

This volume explores the techniques used to study human and animal pericytes, including pericyte dissociation, immunohistochemistry, flow cytometry, avian chimaeras, purification, tissue turnover, and cancer dissemination. It is written in the Methods in Molecular Biology series format and is a valuable resource for researchers.

Format: Paperback / softback
Length: 192 pages
Publication date: 13 February 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into the diverse techniques employed to study human and animal pericytes. Spanning a wide range of topics, the chapters in this volume explore various aspects, including the detachment of pericytes from tissues, their analysis through immunohistochemistry and flow cytometry, the utilization of avian chimaeras to investigate the embryonic development of cell lineage, the purification of pericytes using fluorescence-activated cell sorting and subsequent culture, the study of tissue turnover and regeneration, and the dynamic investigation of pericyte involvement in cancer dissemination, both in situ and in culture. Written in the renowned Methods in Molecular Biology series format, each chapter includes an introduction that provides a concise overview of the respective topic, a comprehensive list of the necessary materials and reagents, detailed step-by-step laboratory protocols that are readily reproducible, and helpful tips on troubleshooting and avoiding common pitfalls. Authored by esteemed experts in the field, Pericytes: Methods and Protocols serves as an invaluable resource for researchers seeking to define the biological identity, functions, and potential therapeutic applications of this increasingly important cell lineage.

Weight: 397g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071610589
Edition number: 1st ed. 2021