Plant Genotyping: Methods and Protocols

This book provides a comprehensive overview of plant genotyping methods, including DNA microarray, DNA sequencing, PCR, and qPCR, and is written for the Methods in Molecular Biology series. It offers step-by-step protocols, troubleshooting tips, and lists of necessary materials and reagents.

Format: Hardback
Length: 469 pages
Publication date: 14 February 2023
Publisher: Springer-Verlag New York Inc.

This comprehensive volume presents a diverse array of existing methods, ranging from the widely adopted to the more unconventional, in the field of plant genotyping. Initially developed for medical research, all genotyping techniques must be applicable across the broad spectrum of plant biology studies, as exemplified in this collection. Plant genotyping methodologies herein rely on various assessments, including DNA microarray, capable of conducting hundreds of thousands of simultaneous reactions, or separate individual studies of DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, digestion with restriction endonucleases, microscopy, and numerous other techniques. Authored for the esteemed Methods in Molecular Biology series, chapters encompass introductions to their respective topics, comprehensive lists of the necessary materials and reagents, detailed step-by-step and readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genotyping: Methods and Protocols provides researchers with the opportunity to enhance their knowledge and select the most suitable method of plant genotyping for their specific application.

Weight: 1107g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071630235
Edition number: 1st ed. 2023