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Phetole Mangena

Plant Transformation via Agrobacterium Tumefaciens: Culture Conditions, Recalcitrance and Advances in Soybean

Plant Transformation via Agrobacterium Tumefaciens: Culture Conditions, Recalcitrance and Advances in Soybean

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  • More about Plant Transformation via Agrobacterium Tumefaciens: Culture Conditions, Recalcitrance and Advances in Soybean


Plant Transformation via Agrobacterium Tumefaciens is a comprehensive guide to the in vitro soybean transformation method,which is a preferred and reliable way to express transgenes in leguminous and non-legume crops. The book covers the history, challenges, types of explants, and applications of transgenic plants for stress resistance, animal/human health, and the preservation of orphan legume crops. It serves as a valuable resource for researchers and students.

Format: Hardback
Length: 226 pages
Publication date: 02 September 2022
Publisher: Taylor & Francis Ltd


Plant Transformation via Agrobacterium Tumefaciens is a comprehensive guide that provides essential and detailed information and procedures related to in vitro soybean transformation, serving as the foundation for Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method is widely recognized as one of the most preferred, reliable, and cost-effective mechanisms for transgene expression in both leguminous recalcitrant species and non-legume crops. Its simplicity, feasibility, and high transformation rates, primarily achieved in monocot plants and a few dicot genotypes, make it a technology of choice for researchers seeking to overcome protocol-related challenges.

The book encompasses a wide range of topics, including the history and discovery of Agrobacterium bacteria, the longstanding challenges associated with transformation inefficiencies, the types and conditions of explants used in transformation, the development of transgenic plants for stress resistance, and the role of transgenic plants in animal/human health, including environmental implications. By providing a comprehensive review of plant transformation, this book serves as a valuable resource for researchers who are still facing protocol-related hurdles.

One of the key themes addressed in Plant Transformation via Agrobacterium Tumefaciens is the risk of overexploitation faced by soybean, and other orphan legume crops, due to their narrow gene pool. The book highlights the importance of plant transformation in improving the gene pool of these crops, enabling the development of varieties that are drought tolerant, have enhanced nutritional value, are pest resistant, and reduce the destruction caused by disease-causing microorganisms.

Moreover, the book emphasizes the role of transgenic plants in addressing global challenges such as food security, climate change, and environmental sustainability. Transgenic plants have the potential to enhance crop yields, improve soil quality, and reduce the use of harmful pesticides and fertilizers. They can also play a crucial role in developing new medicines and therapies for human and animal health.

In conclusion, Plant Transformation via Agrobacterium Tumefaciens is an essential foundation tool that is available to researchers and students seeking to advance their knowledge and understanding of plant transformation. The book provides comprehensive coverage of the topic, covering both fundamental principles and specific procedures, and serves as a valuable resource for those working in the field of plant genetics and biotechnology.

Weight: 610g
Dimension: 234 x 156 (mm)
ISBN-13: 9781032250373

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