Plant Transformation via Agrobacterium Tumefaciens: Culture Conditions, Recalcitrance and Advances in Soybean

Plant Transformation via Agrobacterium Tumefaciens is a comprehensive guide to the in vitro soybean transformation method,which is a preferred and reliable way to express transgenes in leguminous and non-legume crops. The book covers the history, challenges, types of explants, and applications of transgenic plants for stress resistance, animal/human health, and the preservation of orphan legume crops. It serves as a valuable resource for researchers and students.

Format: Paperback / softback
Length: 226 pages
Publication date: 01 September 2022
Publisher: Taylor & Francis Ltd

Plant Transformation via Agrobacterium Tumefaciens is a comprehensive guide that provides essential and detailed information and procedures related to in vitro soybean transformation, serving as the foundation for Agrobacterium-mediated genetic manipulation of soybean through plant tissue culture. This method is widely recognized as one of the most preferred, reliable, and cost-effective mechanisms for transgene expression in both leguminous recalcitrant species and non-legume crops. Its simplicity, feasibility, and high transformation rates, primarily achieved in monocot plants and a few dicot genotypes, make it a technology of choice for researchers seeking to overcome protocol-related challenges.

The book encompasses a wide range of topics, including the history and discovery of Agrobacterium bacteria, the longstanding challenges associated with transformation inefficiencies, the types and conditions of explants used in transformation, the development of transgenic plants for stress resistance, and the role of transgenic plants in animal/human health, including environmental implications. By providing a comprehensive review of plant transformation, this book serves as a valuable resource for researchers who are still facing protocol-related hurdles.

One of the key themes addressed in Plant Transformation via Agrobacterium Tumefaciens is the risk of overexploitation faced by soybean, and other orphan legume crops, due to their narrow gene pool. The book emphasizes the importance of plant transformation in improving the gene pool, thereby developing varieties that are drought tolerant, have enhanced nutritional value, are pest resistant, and reduce the destruction caused by disease-causing microorganisms. This approach is crucial in sustaining the productivity and sustainability of these crops, particularly in the face of climate change and other environmental challenges.

The book is organized into several chapters, each covering a specific aspect of plant transformation. The first chapter provides an introduction to Agrobacterium bacteria and their role in plant transformation. It discusses the history of Agrobacterium discovery, the mechanisms of infection, and the various techniques used for transformation, including binary vector systems, agroinfiltration, and transient expression. The second chapter focuses on the development of transgenic plants for stress resistance. It explores the use of Agrobacterium-mediated transformation to introduce genes encoding stress-related proteins, such as heat shock proteins, drought-tolerant enzymes, and herbicide resistance, into soybean and other crop plants. The third chapter discusses the role of transgenic plants in animal/human health. It explores the potential benefits and risks associated with the consumption of transgenic crops, including the impact on food safety, nutrition, and environmental sustainability. The fourth chapter discusses the environmental implications of plant transformation. It examines the potential impact of transgenic plants on biodiversity, ecosystem functioning, and pesticide resistance, and explores strategies for mitigating these effects.

Throughout the book, numerous case studies and examples are provided to illustrate the practical applications of plant transformation. These examples cover a wide range of crops, including soybean, rice, maize, and cotton, and demonstrate the successful integration of transgenic traits into these species. The book also includes detailed protocols and troubleshooting tips, which are essential for researchers who are new to plant transformation.

In conclusion, Plant Transformation via Agrobacterium Tumefaciens is a comprehensive and essential guide for researchers and students interested in plant transformation. It provides a thorough understanding of the principles, techniques, and applications of plant transformation, and serves as a valuable resource for overcoming protocol-related hurdles and developing transgenic plants with desired traits. By addressing the risk of overexploitation, improving the gene pool, and addressing the environmental implications of plant transformation, this book contributes to the sustainability and productivity of agriculture and other fields.

Weight: 450g
Dimension: 234 x 156 (mm)
ISBN-13: 9781032250380