Radiation Oncology and Radiotherapy, Part A

The Methods in Cell Biology series, Radiation Oncology and Radiotherapy, Volume 172, highlights advances in the field, including DNA damage quantification by the COMET assay, Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells, Immunoblotting-based characterization of the DNA damage response, Assessment of lipid peroxidation in irradiated cells, and more. Other sections cover Assessment of transcription inhibition as a characteristic of immunogenic cell death, Assessment of eIF2a phosphorylation during immunogenic cell death, Quantification of cytosolic DNA species by immunofluorescence and automated image analysis, Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death, and Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells.

Format: Hardback
Length: 214 pages
Publication date: 01 September 2022
Publisher: Elsevier Science & Technology

Radiation Oncology and Radiotherapy,Part A,Volume 172 in the Methods in Cell Biology series,presents a comprehensive overview of advancements in the field. This latest volume offers engaging chapters on timely topics, such as DNA damage quantification by the COMET assay,Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells,Immunoblotting-based characterization of the DNA damage response,Assessment of lipid peroxidation in irradiated cells,A simple method to assess clonogenic survival of irradiated cancer cells,Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence,Cytofluorometric assessment of cell cycle progression in irradiated cells,and much more. Additionally, other sections delve into Assessment of transcription inhibition as a characteristic of immunogenic cell death,Assessment of eIF2a phosphorylation during immunogenic cell death,Quantification of cytosolic DNA species by immunofluorescence and automated image analysis,Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death,Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells,and numerous other topics of interest.

Radiation Oncology and Radiotherapy,Part A,Volume 172 in the Methods in Cell Biology series,presents a comprehensive overview of advancements in the field. This latest volume offers engaging chapters on timely topics, such as DNA damage quantification by the COMET assay,Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells,Immunoblotting-based characterization of the DNA damage response,Assessment of lipid peroxidation in irradiated cells,A simple method to assess clonogenic survival of irradiated cancer cells,Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence,Cytofluorometric assessment of cell cycle progression in irradiated cells,and much more. Additionally, other sections delve into Assessment of transcription inhibition as a characteristic of immunogenic cell death,Assessment of eIF2a phosphorylation during immunogenic cell death,Quantification of cytosolic DNA species by immunofluorescence and automated image analysis,Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death,Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells,and numerous other topics of interest.

Weight: 578g
Dimension: 201 x 242 x 18 (mm)
ISBN-13: 9780323899499