RNA Methodologies: A Laboratory Guide for Isolation and Characterization

Sixth Edition of RNA Methodologies: A Laboratory Guide for Isolation and Characterization provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students. It covers RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes, and bioinformatics, with new material on these topics and advances in methods and protocols.

Format: Paperback / softback
Length: 960 pages
Publication date: 01 October 2022
Publisher: Elsevier Science & Technology

Sixth Edition of RNA Methodologies: A Laboratory Guide for Isolation and Characterization provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology.

RNA sequencing has revolutionized our understanding of gene expression and regulation, allowing us to rapidly and accurately sequence entire genomes and transcriptomes. This technique has opened up new avenues for research in fields such as genetics, epigenetics, and cancer biology. RNA in Situ Hybridization (ISH) is a powerful technique used to detect and localize RNA molecules in cells and tissues. It allows researchers to visualize the location and abundance of specific RNA species, providing valuable insights into gene expression and regulation. Non-coding RNAs, such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), play critical roles in gene expression and regulation. These RNAs are often involved in epigenetic regulation, tumor suppression, and immune response. Computational RNA biology is an emerging field that uses computer science and mathematics to study RNA molecules and their interactions. It allows researchers to predict RNA structures, identify regulatory elements, and analyze RNA-protein interactions. Transcriptomes and bioinformatics are essential tools for studying gene expression and regulation. They provide a comprehensive view of the RNA molecules present in a cell or organism, allowing researchers to identify differentially expressed genes, gene expression patterns, and functional annotations.

In recent years, there have been significant advances in RNA isolation and purification techniques, such as magnetic bead-based isolation and microfluidic devices. These techniques have improved the efficiency and specificity of RNA isolation, making it easier to obtain high-quality RNA for further analysis.

In conclusion, RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition is a comprehensive and up-to-date resource for researchers working with RNA in the laboratory. It provides essential knowledge and techniques for RNA isolation, characterization, and analysis, and covers the latest advances in RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes, and bioinformatics. With the expertise of Dr. Farrell and his personal experience in the field, this book is an invaluable tool for both experienced practitioners and beginners in the field of RNA biology.

Dimension: 229 x 152 (mm)
ISBN-13: 9780323902212
Edition number: 6 ed