Separation Techniques Applied to Omics Sciences: From Principles to Relevant Applications

This book covers liquid chromatography, gas chromatography, and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics, and foodomics. It presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment.

Format: Paperback / softback
Length: 273 pages
Publication date: 10 October 2022
Publisher: Springer Nature Switzerland AG

This comprehensive book delves into the realm of liquid chromatography, gas chromatography, and capillary electrophoresis, three cutting-edge separation techniques that have emerged as pivotal in key omic sciences such as genomics, proteomics, metabolomics, and foodomics. While it does not delve into the fundamentals of each technique, it instead focuses on presenting the latest advancements in these methods, emphasizing their applications in omics analyses and their unique characteristics. The primary objective of this volume is to provide researchers with a diverse range of options available in the field of omics sciences and to guide them on how to effectively integrate these approaches to gain a comprehensive understanding of biological systems.

Omic sciences have assumed an indispensable role in unraveling the intricate biochemical reactions and associated events that occur within biological systems. The traditional central dogma of molecular biology, which posits a unidirectional flow of genetic information from DNA to RNA and subsequently to proteins, has gradually given way to the systems biology approach. This book adopts a multidisciplinary perspective, elucidating the biological system as a holistic entity, wherein the entire organism is influenced by a multitude of internal events as well as by its external environment. It underscores the interconnectedness of each level of biological information flux, highlighting how changes at one level can impact the subsequent ones.

Liquid chromatography, gas chromatography, and capillary electrophoresis are powerful tools employed in omics analyses to separate, identify, and quantitate various biomolecules. In this book, we explore their applications in genomics, proteomics, metabolomics, and foodomics, highlighting their strengths and limitations. We discuss the techniques used for sample preparation, separation, and data analysis, emphasizing the importance of quality control and reproducibility. Additionally, we explore the emerging trends and advancements in these techniques, such as high-throughput screening, single-cell analysis, and mass spectrometry, which are enabling researchers to uncover new insights into complex biological processes.

In conclusion, this comprehensive book provides researchers with a valuable resource for understanding and leveraging the power of liquid chromatography, gas chromatography, and capillary electrophoresis in omics sciences. By presenting the latest advancements and applications in these techniques, it empowers researchers to explore the vast landscape of biological systems and gain a deeper understanding of the complex interplay between biomolecules and biological processes.

Weight: 552g
Dimension: 254 x 178 (mm)
ISBN-13: 9783030772543
Edition number: 1st ed. 2021