Sepsis: Methods and Protocols

This book provides detailed animal models and key procedures to measure specific disease outcomes in sepsis research. It includes surgical and nonsurgical rodent models, protocols for burn injury and sepsis, humanized mice, and biomarkers for diagnosis and prognosis. Agent-based computational modeling is also presented as a valuable approach. Written for researchers, the book offers step-by-step protocols and troubleshooting tips.

Format: Paperback / softback
Length: 262 pages
Publication date: 30 May 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume offers a diverse range of animal models commonly employed in studying sepsis, along with essential procedures to assess specific disease outcomes. The chapters provide detailed descriptions of well-established surgical and nonsurgical rodent models of sepsis, presented by esteemed experts in the field. Additionally, the book encompasses protocols for burn injury and sepsis, modeling the mouse intensive care unit (MICU), and the development of humanized mice, which hold potential as valuable tools to enhance the translational potential of rodent sepsis research. A dedicated chapter explores the utilization of biomarkers for diagnosing and predicting the prognosis of sepsis in humans and mice, while other chapters delve into specific methods for measuring biomarkers and the use of analgesics and humane endpoints in rodent sepsis studies. Furthermore, agent-based computational modeling is presented as a valuable complementary approach to studying sepsis. Authored for the esteemed Methods in Molecular Biology series, the chapters feature introductions to their respective topics, comprehensive lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and helpful tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Sepsis: Methods and Protocols serves as an invaluable guide for researchers seeking to enhance the identification and treatment of septic patients.

Weight: 520g
Dimension: 176 x 253 x 22 (mm)
ISBN-13: 9781071614907
Edition number: 1st ed. 2021