Serum/Plasma Proteomics: Methods and Protocols

This third volume of the "Serum/Plasma Proteomics: Methods and Protocols" series provides comprehensive protocols for pre-analytical, analytical, plasma, and serum proteomics, including blood processing and handling strategies, discovery- and targeted-based mass spectrometry, software and bioinformatics, enrichment and detection strategies, peptide, lipid, and metabolite targeted assays, and the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful "Methods in Molecular Biology" series, the book aims to be a comprehensive guide for researchers.

Format: Hardback
Length: 596 pages
Publication date: 14 February 2023
Publisher: Springer-Verlag New York Inc.

This third volume in the series provides comprehensive protocols for pre-analytical, analytical, plasma, and serum proteomics. It includes new and updated chapters organized into nine sections, covering various aspects of blood processing and handling strategies, discovery- and targeted-based mass spectrometry, workflows for data analysis, software and bioinformatics for plasma proteomics, emerging technologies for plasma proteomics, assay platforms for biomarker discovery, enrichment and detection strategies, peptide, lipid, and metabolite targeted assays, and the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of necessary materials and methods, troubleshooting tips, and step-by-step, readily reproducible protocols. Authored by renowned experts in the field, the book aims to serve as a comprehensive guide for researchers seeking to advance their knowledge and expertise in serum/plasma proteomics.

Introduction:
Serum/Plasma Proteomics: Methods and Protocols, Third Edition, is a comprehensive and authoritative guide for researchers seeking to explore the complex world of proteomics in serum and plasma samples. This volume provides detailed protocols and methodologies for pre-analytical, analytical, plasma, and serum proteomics, covering a wide range of techniques and applications.

Chapter Organization:
The book is organized into nine sections, each dedicated to a specific aspect of serum/plasma proteomics. These sections include:

• Pre-analytical Considerations:

This section covers topics such as sample preparation, blood collection, and storage, emphasizing the importance of proper handling and preservation of samples to ensure accurate and reliable proteomic analysis.

• Analytical Methods:

This section discusses the various analytical techniques used in serum/plasma proteomics, including mass spectrometry-based methods such as shotgun proteomics, targeted proteomics, and quantitative proteomics. It also covers methods for protein separation, sample cleanup, and data analysis.

• Plasma Proteomics:

This section focuses on the study of proteins in plasma, including their composition, dynamics, and potential biomarkers. It covers methods for plasma sample preparation, protein isolation, quantification, and analysis of plasma proteomes.

• Serum Proteomics:

This section explores the study of proteins in serum, including their composition, dynamics, and potential biomarkers. It covers methods for serum sample preparation, protein isolation, quantification, and analysis of serum proteomes.

• Emerging Areas:

This section covers emerging areas in serum/plasma proteomics, such as the analysis of extracellular vesicles, microRNAs, and other non-protein molecules. It provides protocols and methodologies for the isolation, purification, and analysis of these molecules.

• Assay Platforms:

This section discusses the development of assay platforms for serum/plasma proteomics, including microarrays, bead-based assays, and high-throughput sequencing technologies. It provides protocols and methodologies for the design, development, and validation of these platforms.

• Software and Bioinformatics:

This section discusses the use of software and bioinformatics tools in serum/plasma proteomics. It covers methods for data management, visualization, and statistical analysis of proteomic data, as well as the integration of proteomic data with other omics data for comprehensive analysis.

• Conclusion:

In conclusion, Serum/Plasma Proteomics: Methods and Protocols, Third Edition, is a valuable resource for researchers and scientists interested in advancing their knowledge and expertise in serum/plasma proteomics. With its comprehensive coverage of protocols, techniques, and applications, this book provides a solid foundation for researchers to explore the vast potential of proteomics in clinical and biological research.

Weight: 1339g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071629772
Edition number: 3rd ed. 2023