Spectral and Imaging Cytometry: Methods and Protocols

Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a comprehensive guide to the latest approaches in spectral cytometry, covering protocols for imaging cytometry using Imagestream technology and introducing the FlowCam cytometer for analyzing phytoplankton and zooplankton. Written for the Methods in Molecular Biology series, the book includes detailed introductions, lists of materials and reagents, and reproducible laboratory protocols with troubleshooting tips and recommendations.

Format: Hardback
Length: 260 pages
Publication date: 20 April 2023
Publisher: Springer-Verlag New York Inc.

This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations. Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resource for beginners and professionals interested in learning more about this developing field.

Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spectral cytometry is a rapidly evolving field that has seen significant advancements in recent years. This second edition volume of Spectral and Imaging Flow Cytometry: Methods and Protocols provides a comprehensive overview of the latest approaches and techniques used in this field.

The book begins with a brief history of spectral cytometry development, providing an introduction to the principles and technologies that underpin this technique. It then moves on to discuss new protocols in different areas of imaging cytometry, including cell sorting, immunophenotyping, and imaging of live cells.

One of the key features of this book is its focus on the unique technology of Imagestream, which is a high-speed imaging cytometer that enables the analysis of large numbers of cells in real time. The book includes detailed protocols for using Imagestream to analyze a wide range of cell types, including bacteria, yeast, and mammalian cells.

In addition to discussing new protocols, the book also introduces FlowCam, a cytometer with capabilities of analyzing phytoplankton and zooplankton. FlowCam uses a combination of flow cytometry and imaging techniques to analyze these organisms, providing valuable insights into their biology and ecology.

The book is written for the highly successful Methods in Molecular Biology series, which is known for its clear and concise writing style and comprehensive coverage of molecular biology techniques. Each chapter includes a brief introduction to its respective topic, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations.

Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resource for beginners and professionals interested in learning more about this developing field. Whether you are a researcher, scientist, or student, this book will provide you with the knowledge and skills necessary to perform spectral and imaging flow cytometry experiments and analyze your data effectively.

Weight: 722g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071630198
Edition number: 2nd ed. 2023