The Immunological Synapse - Part B

The Methods in Cell Biology series' Immunological Synapse - Part B,Volume 178 offers cutting-edge techniques for studying T cell and natural killer (NK) cell synapses, such as imaging polarized granule release, analyzing actin reorganization, and quantifying interactions. It also covers proteomic analysis, NK-tumor cell interaction, CAR-modified cell immunological synapse quality, and potency monitoring of therapeutic CAR T cells.

Format: Hardback
Length: 318 pages
Publication date: 14 July 2023
Publisher: Elsevier Science Publishing Co Inc

The Immunological Synapse - Part B,Volume 178 in the Methods in Cell Biology series is a comprehensive resource for studying the intricate mechanisms of the immunological synapse. This first volume delves into diverse aspects of T cell and natural killer (NK) cell synapses, encompassing imaging polarized granule release using TIRF microscopy, analyzing actin reorganization and centrosome polarization, investigating redirected degranulation, employing live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantifying interactions between antigen-presenting cells (APCs) and T cells, assessing the membrane lipid state at the immunological synapse, conducting proteomic analysis and imaging of NK-tumor cell interactions, evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue, examining the quality of chimeric antigen receptor (CAR)-modified cell immunological synapses using the glass-supported planar lipid bilayer, and monitoring the potency of therapeutic CAR T cells. Additionally, the book includes two chapters on quantifying lymphocytic choriomeningitis virus (LCMV)-specific T cells and LCMV viral titers, as well as one chapter on the murine antibody-dependent cellular phagocytosis assay.

The Immunological Synapse - Part B,Volume 178 in the Methods in Cell Biology series is a comprehensive resource for studying the intricate mechanisms of the immunological synapse.

This first volume delves into diverse aspects of T cell and natural killer (NK) cell synapses, encompassing imaging polarized granule release using TIRF microscopy, analyzing actin reorganization and centrosome polarization, investigating redirected degranulation, employing live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantifying interactions between antigen-presenting cells (APCs) and T cells, assessing the membrane lipid state at the immunological synapse, conducting proteomic analysis and imaging of NK-tumor cell interactions, evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue, examining the quality of chimeric antigen receptor (CAR)-modified cell immunological synapses using the glass-supported planar lipid bilayer, and monitoring the potency of therapeutic CAR T cells.

Additionally, the book includes two chapters on quantifying lymphocytic choriomeningitis virus (LCMV)-specific T cells and LCMV viral titers, as well as one chapter on the murine antibody-dependent cellular phagocytosis assay.

Weight: 450g
Dimension: 229 x 152 (mm)
ISBN-13: 9780443191633