The Integrated Stress Response: Methods and Protocols

This book provides an up-to-date collection of protocols for investigating the integrated stress response (ISR), a vital mechanism for eukaryotic cells to adapt to stress. It covers methods for analyzing mRNA translation regulation and interaction networks and ribonucleoprotein (RNP) granules. Written for the Methods in Molecular Biology series, the protocols are highly reproducible and include troubleshooting tips.

Format: Hardback
Length: 404 pages
Publication date: 17 February 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume presents an up-to-date compilation of protocols detailing essential approaches for investigating the integrated stress response (ISR), a vital evolutionary conserved mechanism that enables eukaryotic cells to adapt to stress conditions and modify their gene expression programs. The book's content is divided into two sections: one focusing on techniques to analyze mRNA translation regulation, and the other on methods to examine interaction networks and ribonucleoprotein (RNP) granules. Authored by renowned experts in the field, each chapter includes an introduction providing a brief overview of the respective topic, a comprehensive list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding common pitfalls. Designed as a valuable resource for researchers and scholars engaged in studying the ISR, The Integrated Stress Response: Methods and Protocols serves as an ideal guide to facilitate rapid progress in this complex and captivating field of biology.

Weight: 976g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071619742
Edition number: 1st ed. 2022