The Orchid Genome

This book provides information on genome complexity and evolution, transcriptome analysis, miRNome, simple sequence repeats, genome relationships, molecular cytogenetics, polyploidy induction and application, flower and embryo development, and the composition, expression, and function of various microRNAs and simple sequence repeats in orchids. It will aid in the efficient development of new cultivars and functional genomics studies.

Format: Paperback / softback
Length: 162 pages
Publication date: 22 April 2022
Publisher: Springer Nature Switzerland AG

This comprehensive book delves into the intricate world of genome complexity and evolution, transcriptome analysis, miRNome, simple sequence repeats, genome relationships, molecular cytogenetics, polyploidy induction, and application in flower and embryo development. Orchids, renowned for their exquisite beauty and significant role in the global floriculture trade, occupy a remarkable position, accounting for approximately 10% of the global fresh cut flower trade. By gaining a deeper understanding of the fundamental botanical characteristics, flower regulation, molecular cytogenetics, karyotypes, and DNA content of these remarkable plants, researchers and breeders can strive to develop new cultivars with enhanced qualities. Moreover, the book explores the composition, expression, and functional roles of various microRNAs and simple sequence repeats, providing valuable insights into their involvement in all aspects of plant growth and development. This knowledge will undoubtedly contribute to the field of functional genomics studies, shedding light on the molecular mechanisms underlying plant development and evolution.

Genome Complexity and Evolution:
The book delves into the study of genome complexity and evolution, exploring the mechanisms and processes that shape the genetic makeup of organisms. It discusses the various factors contributing to genome evolution, such as gene duplication, deletion, and rearrangement, as well as the role of natural selection in shaping the diversity of life on Earth. The book also provides insights into the techniques used to study genome structure and function, including DNA sequencing, bioinformatics, and genetic mapping.

Transcriptome Analysis:
In this chapter, the book discusses the methods and techniques used to analyze the transcriptome, the entire set of RNA molecules produced by an organism. It explores the role of transcripts in gene expression, regulation, and cellular processes, and highlights the applications of transcriptome analysis in understanding plant development, disease resistance, and environmental adaptation. The book also discusses the use of next-generation sequencing technologies to rapidly and efficiently sequence large transcriptomes.

miRNome:
The miRNome, or microRNAome, is a crucial component of gene regulation in plants. The book explores the structure, function, and discovery of microRNAs in orchids and other plant species. It discusses the mechanisms by which microRNAs regulate gene expression, their involvement in developmental processes, and their potential applications in crop improvement and disease control. The book also provides insights into the techniques used to identify and characterize microRNAs, including microarray analysis and next-generation sequencing.

Simple Sequence Repeats:
Simple sequence repeats, or SSRs, are DNA sequences that occur frequently throughout the genome. The book discusses the role of SSRs in genome structure, evolution, and genetic diversity. It explores the methods used to identify and characterize SSRs, including PCR-based techniques and bioinformatics tools. The book also discusses the applications of SSRs in genetic mapping, marker-assisted breeding, and population genetics.

Genome Relationships:
The book explores the relationships between different genomes within a species or across species. It discusses the methods used to identify and analyze genetic similarities and differences, such as DNA sequencing, hybridization, and phylogenetic analysis. The book also provides insights into the evolutionary processes that drive genome relationships, including speciation, gene flow.

Molecular Cytogenetics:
Molecular cytogenetics is a branch of genetics that studies the structure, organization, and function of chromosomes in plants. The book discusses the methods and techniques used to study the cytogenetics of orchids, including chromosome preparation, chromosome staining, and fluorescence in situ hybridization. It highlights the applications of molecular cytogenetics in understanding genome structure, gene expression, and genetic diversity.

Polyploidy Induction and Application:
Polyploidy is a process in which a plant cell gains multiple copies of its genome. The book discusses the methods and techniques used to induce polyploidy in orchids, including somatic hybridization, tissue culture, and genetic transformation. It explores the applications of polyploidy in crop improvement, disease resistance, and ornamental plant breeding. The book also discusses the challenges and limitations associated with polyploidy induction and the need for careful management to avoid unintended consequences.

Flower and Embryo Development:
Orchids are known for their exquisite flowers and complex embryogenesis. The book discusses the developmental processes and mechanisms involved in flower and embryo formation in orchids. It explores the role of hormones, genes, and environmental factors in regulating flower development, as well as the molecular mechanisms underlying embryogenesis. The book also provides insights into the techniques used to study flower and embryo development, including microscopy, tissue culture, and genetic manipulation.

Weight: 353g
Dimension: 254 x 178 (mm)
ISBN-13: 9783030668280
Edition number: 1st ed. 2021