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Thermogenic Fat: Methods and Protocols
Thermogenic Fat: Methods and Protocols
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- More about Thermogenic Fat: Methods and Protocols
This volume provides a comprehensive guide to studying thermogenic fat biology using in vitro cell culture and animal models. It covers protocols for differentiating human pluripotent stem cells and murine adipocyte precursors, measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism. It also includes techniques for AAV-mediated gene delivery, adipose tissue transplantation, isolation of adipose tissue immune cells, and mass spectrometry-based profiling of brown fat lipids. Written in the format of the highly successful Methods in Molecular Biology series, the book aims to be a valuable resource for researchers in the field.
Format: Hardback
Length: 253 pages
Publication date: 20 April 2023
Publisher: Springer-Verlag New York Inc.
This comprehensive volume offers a diverse range of state-of-the-art approaches to studying the biology of thermogenic fat through in vitro cell culture and animal models. The chapters provide detailed protocols for differentiating human pluripotent stem cells and murine adipocyte precursors, as well as methods for measuring mitochondrial respiration, heat generation, brown fat activation, and the impact on energy metabolism in mice. Additionally, techniques for AAV-mediated gene delivery, adipose tissue transplantation, isolation of adipose tissue immune cells and extracellular vesicles, and mass spectrometry-based profiling of brown fat lipids are covered. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of necessary materials and methods, tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authored by esteemed experts in the field, Thermogenic Fat: Methods and Protocols strives to be an indispensable guide for researchers seeking to delve into the complexities of thermogenic fat research.
Weight: 707g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071631669
Edition number: 1st ed. 2023
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