Transgenesis: Methods and Protocols

This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. CRISPR technology supersedes earlier methods that relied on random transgene integration, and the book is written for the highly successful Methods in Molecular Biology series. It serves as an ideal guide for researchers working with or working on genetically modified models.

Format: Hardback
Length: 448 pages
Publication date: 31 March 2023
Publisher: Springer-Verlag New York Inc.

This extensive volume delves into genotyping and validation, encompassing detailed insights into the production of gene-edited cells and animals for research purposes. As CRISPR technology emerges as a superior method for precise gene targeting in the genome, it holds immense potential for advancing biomedical research. This collection, spanning the highly successful Methods in Molecular Biology series, offers a comprehensive exploration of various transgenesis techniques. Each chapter provides an introduction to its respective topic, accompanied by a comprehensive list of necessary materials and reagents. Step-by-step and readily reproducible laboratory protocols are presented, along with practical tips for troubleshooting and avoiding common pitfalls. Authored by esteemed experts in the field, Transgenesis: Methods and Protocols serves as an invaluable guide for researchers engaged in or embarking upon work with genetically modified models. Its authoritative and practical nature makes it an ideal resource for scholars and practitioners seeking to advance their understanding and applications of transgenesis in the realm of biomedical research.

Weight: 1056g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071629895
Edition number: 1st ed. 2023