Trophoblasts: Methods and Protocols

This volume explores the latest approaches used to assess trophoblast angiogenesis, transport function, cellular respirations, migration, and invasion, covering topics such as primary trophoblast cell studies, liposome-based biomolecule delivery, in vitro placenta models, and heterogenous cell types and 3D-organoid structures. It is a valuable resource for researchers in the field of developmental origins of health and diseases (DoHaD).

Format: Hardback
Length: 248 pages
Publication date: 30 November 2023
Publisher: Springer-Verlag New York Inc.

Trophoblasts: Methods and Protocols is a comprehensive volume that delves into the latest approaches used to assess trophoblast angiogenesis, transport function, cellular respirations, migration, and invasion. The book covers various methods to study and manipulate primary trophoblast cells, strategies using liposomes to deliver biomolecules to trophoblasts, in vitro models of the placenta that emulate the cellular interactions in the 3D uterine environment, and models exploring heterogenous cell types and 3D-organoid structures that represent cell-to-cell interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

This volume is a valuable resource for researchers who want to better understand the function of the placenta and its complex contribution to maternal health and fetal development. It also provides greater insights into the area of study known as the developmental origins of health and diseases (DoHaD).

The book begins with an overview of the placenta and its role in pregnancy. It then discusses the various methods used to study trophoblasts, including in vitro models, animal models, and human studies. The chapters cover topics such as trophoblast angiogenesis, transport function, cellular respirations, migration, and invasion.

One of the key features of the book is the use of liposomes to deliver biomolecules to trophoblasts. Liposomes are small, lipid-based vesicles that can be used to transport drugs, proteins, and other molecules into cells. The book provides detailed protocols for the preparation and use of liposomes, as well as examples of their use in various research applications.

In addition to liposomes, the book also covers in vitro models of the placenta. These models are designed to emulate the cellular interactions in the 3D uterine environment. The models include co-culture systems, organoid cultures, and microfluidic devices. These models allow researchers to study the behavior of trophoblasts in a more realistic and controlled environment.

The book also includes chapters on heterogenous cell types and 3D-organoid structures. These structures represent cell-to-cell interactions in vivo and can be used to study the development of the placenta and other tissues. The chapters provide detailed protocols for the preparation and use of these structures, as well as examples of their use in various research applications.

One of the challenges in studying trophoblasts is the heterogeneity of the cell population. Trophoblasts can be divided into different subtypes, each with its own unique characteristics and functions. The book provides detailed protocols for the isolation and characterization of these subtypes, as well as examples of their use in various research applications.

Another challenge in studying trophoblasts is the lack of appropriate animal models. Animal models can be used to study the development of the placenta and other tissues, but they are not always accurate representations of human physiology. The book provides detailed protocols for the use of animal models in trophoblast research, as well as examples of their use in various research applications.

In addition to the laboratory protocols, the book also includes tips on troubleshooting and avoiding known pitfalls. The authors provide detailed explanations of common errors and how to avoid them, as well as examples of successful experiments.

Overall, Trophoblasts: Methods and Protocols is a valuable resource for researchers who want to better understand the function of the placenta and its complex contribution to maternal health and fetal development. It provides detailed protocols for the study of trophoblasts, as well as examples of their use in various research applications. The book is written in the highly successful Methods in Molecular Biology series format, which makes it easy to read and follow.

In conclusion, Trophoblasts: Methods and Protocols is a comprehensive volume that delves into the latest approaches used to assess trophoblast angiogenesis, transport function, cellular respirations, migration, and invasion. The book covers various methods to study and manipulate primary trophoblast cells, strategies using liposomes to deliver biomolecules to trophoblasts, in vitro models of the placenta that emulate the cellular interactions in the 3D uterine environment, and models exploring heterogenous cell types and 3D-organoid structures that represent cell-to-cell interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. This volume is a valuable resource for researchers who want to better understand the function of the placenta and its complex contribution to maternal health and fetal development, and also provide greater insights to the area of study known as the developmental origins of health and diseases (DoHaD).

Dimension: 254 x 178 (mm)
ISBN-13: 9781071634943
Edition number: 1st ed. 2024