Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols

This second edition includes a collection of antigen production and delivery strategies for vaccine development in veterinary species, with new and updated chapters guiding readers through protocols for antigen production, experimental antigen delivery, and the analysis of immune responses upon vaccination.

Format: Paperback / softback
Length: 306 pages
Publication date: 04 February 2023
Publisher: Springer-Verlag New York Inc.

This second edition encompasses a comprehensive collection of strategies for the production and delivery of vaccines in veterinary species. In addition to new and revised chapters, this edition provides readers with detailed protocols for antigen production, experimental antigen delivery, and the analysis of immune responses following vaccination. Written in the popular format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of necessary materials and reagents, tips on troubleshooting and common pitfalls, and step-by-step, readily reproducible protocols. Authored by esteemed experts in the field, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols, Second Edition serves as an authoritative and cutting-edge resource for researchers seeking to advance their understanding in this critical area.

Antigen production and delivery strategies play a crucial role in vaccine development for veterinary species. In this second edition, the book aims to provide a comprehensive and up-to-date guide to these techniques. The chapters are written in a clear and concise manner, making them accessible to researchers with varying backgrounds.

The introduction to each chapter provides a brief overview of the topic, highlighting its significance and relevance in veterinary medicine. It also outlines the objectives and goals of the respective protocols, which are designed to facilitate the production and delivery of vaccines effectively.

The necessary materials and reagents required for each protocol are extensively listed, along with their suppliers and recommended sources. This information is essential for researchers to ensure that they have access to the required resources and can execute the protocols successfully.

Tips on troubleshooting and known pitfalls are provided throughout the book, helping researchers avoid common errors and improve the efficiency of their experiments. These insights are derived from the authors' extensive experience and expertise in the field.

Step-by-step, readily reproducible protocols are provided for each antigen production and delivery technique. These protocols are designed to be followed by researchers with minimal prior experience in the field. They include detailed instructions, illustrations, and references to relevant literature, ensuring that researchers can reproduce the results accurately.

The book covers a wide range of antigen production and delivery strategies, including recombinant protein production, viral vector-based vaccines, and live attenuated vaccines. Each strategy is discussed in detail, with an emphasis on its advantages, limitations, and potential applications in veterinary medicine.

The analysis of immune responses upon vaccination is also covered in depth. The chapters provide detailed protocols for measuring immune responses, including antibody titers, cytokine profiles, and cellular immune responses. This information is crucial for understanding the efficacy of vaccines and developing improved vaccine formulations.

Overall, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols, Second Edition is a valuable resource for researchers and practitioners in the field of veterinary medicine. It provides a comprehensive and up-to-date guide to antigen production and delivery strategies, covering a wide range of techniques and applications. The book is written in a clear and concise manner, making it accessible to researchers with varying backgrounds. With its step-by-step protocols and troubleshooting tips, it is an essential tool for advancing the study of veterinary viral diseases and developing effective vaccines.

Weight: 610g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071621707
Edition number: 2nd ed. 2022