Alzheimer's Disease: Methods and Protocols

This book explores the latest techniques used to study the human brain in order to understand Alzheimer's Disease and related neurodegenerative disorders. It is written in the highly successful Methods in Molecular Biology series format and includes chapters on post-mortem brain preparations, neural cellular models, nucleic acid analyses, lipid analyses, and protein analyses.

Format: Hardback
Length: 340 pages
Publication date: 19 November 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into the most recent methodologies employed to investigate the human brain, with the ultimate goal of comprehending Alzheimer's Disease and related neurodegenerative disorders. Contributions from esteemed experts worldwide are featured in the chapters of this book, which are organized into five distinct sections. Part One explores the realm of human post-mortem brain preparations, encompassing techniques such as single-cell isolation and the utilization of specialized imaging methods. Part Two delves into the realm of neural cellular models, employing both primary and human-induced pluripotent stem cells to replicate various aspects of the human brain. Part Three focuses on nucleic acid analyses, encompassing transcriptomic and somatic genomic alterations, while Part Four delves into lipid analyses through the use of mass spectrometry. Lastly, Part Five explores protein analyses, with a particular emphasis on the proteins Aβ and Tau. Adhering to the renowned Methods in Molecular Biology series format, chapters in this book feature comprehensive introductions to their respective topics, comprehensive lists of the necessary materials and reagents, detailed step-by-step laboratory protocols that are readily reproducible, and valuable tips on troubleshooting and avoiding common pitfalls. As a cutting-edge and thorough resource, Alzheimer's Disease: Methods and Protocols serves as an invaluable tool for all researchers seeking to broaden their expertise and understanding of this disease and other related dementias.

Weight: 867g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071626542
Edition number: 1st ed. 2023