Animal Cell Culture: Principles and Practice

This guide provides a step-by-step approach to standard animal cell culture techniques, covering lab safety, sterility management, preparation, ethical considerations, and troubleshooting. It is an essential handbook for novice researchers and students in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.

Format: Hardback
Length: 327 pages
Publication date: 02 February 2023
Publisher: Springer International Publishing AG

This comprehensive guide is designed to provide novice researchers and lab students with a thorough and step-by-step approach to standard animal cell culture techniques. It encompasses a wide range of topics, including lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common challenges. Written by experienced researchers and educators, this handbook is an invaluable resource for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and other related fields.

Lab safety and best practices are essential components of animal cell culture. Proper handling, equipment sterilization, and waste disposal are crucial to prevent the spread of infections and maintain a clean and safe working environment. This guide provides detailed instructions on how to handle cells, culture media, and reagents safely, as well as how to set up and maintain a sterile lab environment.

Sterility management is another critical aspect of animal cell culture. It involves maintaining the sterility of cells, culture media, and laboratory equipment to ensure the integrity of the experiment. This guide covers various sterilization techniques, such as heat sterilization, chemical sterilization, and biological sterilization, and provides step-by-step instructions on how to perform them effectively.

Preparation of animal cells for culture is a complex process that requires careful attention to detail. This guide covers the preparation of cell cultures from primary cells, cell lines, and tissue samples, and provides step-by-step instructions on how to isolate, grow, and maintain cells in a controlled environment. It also discusses the importance of cell culture media and additives and how to select and optimize them for specific cell types.

Ethical considerations are an important aspect of animal cell culture. Researchers must ensure that the use of animals in research is ethical, humane, and in accordance with relevant regulations and guidelines. This guide provides information on ethical considerations related to animal cell culture, including the use of animal models, the number of animals used, and the conditions under which they are used.

Troubleshooting is an essential part of animal cell culture. Common problems can arise during the experiment, such as cell growth abnormalities, contamination, and equipment failure. This guide provides troubleshooting tips and techniques to help researchers identify and resolve these problems quickly and efficiently.

In conclusion, this comprehensive guide provides novice researchers and lab students with a thorough and step-by-step approach to standard animal cell culture techniques. It covers lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. By following the guidelines and techniques outlined in this handbook, researchers can ensure the success of their animal cell culture experiments and contribute to advancing scientific knowledge in their respective fields.

Weight: 682g
Dimension: 235 x 155 (mm)
ISBN-13: 9783031194849
Edition number: 1st ed. 2023