Antisense RNA Design, Delivery, and Analysis

This open-access book provides a comprehensive collection of models, delivery systems, and approaches for assessing RNA technology in exploiting antisense as a therapeutic intervention. It is written for the Methods in Molecular Biology series and covers design, efficacy, preclinical toxicity, and intellectual property considerations. The book is authored by experts in the field and is a valuable resource for newcomers and experienced researchers alike.

Format: Paperback / softback
Length: 422 pages
Publication date: 26 February 2022
Publisher: Springer-Verlag New York Inc.

This open-access volume brings together a diverse range of models, delivery systems, and approaches that can be employed to evaluate RNA technology for the therapeutic exploitation of antisense. The book commences with a section dedicated to the design and delivery of antisense agents, followed by a comprehensive exploration of model systems developed to assess their efficacy, both in vivo and in vitro. Additionally, it delves into methods to evaluate the preclinical toxicity associated with these novel potential drugs and addresses intellectual property considerations. Authored by esteemed experts in the field, the chapters in this book encompass introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls.

Designed as a part of the highly successful Methods in Molecular Biology series, the chapters offer insightful overviews, comprehensive lists of required materials and reagents, detailed step-by-step laboratory protocols, and practical guidance on troubleshooting and avoiding common challenges.

Antisense RNA Design, Delivery, and Analysis serves as an authoritative and practical resource, providing foundational knowledge and a vast collection of methods to facilitate the work of newcomers to this dynamic and rapidly evolving field. The book was conceived in collaboration with the DARTER (Delivery of Antisense RNA Therapeutics) network, which is funded by the EU Cooperation of Science and Technology (COST). The primary objective of DARTER is to foster interaction and collaborations among researchers in Europe and other countries, thereby advancing the field of antisense RNA therapeutics.

Weight: 832g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071620120
Edition number: 1st ed. 2022