Apoptosis and Cancer: Methods and Protocols

This volume discusses methods used in molecular and cellular biology for detecting and studying cell death, especially in cancer and cancer therapy. Chapters cover topics such as non-destructive, real-time Annexin V apoptosis assay, assessment of the immune response to tumor cell apoptosis and efferocytosis, mass cytometry assessment of cell phenotypes and signaling states in human whole blood, cell cycle analysis of ER stress and autophagy, and simultaneous detection of inflammasome activation and membrane damage during pyroptosis.

Format: Hardback
Length: 212 pages
Publication date: 11 September 2022
Publisher: Springer-Verlag New York Inc.

This comprehensive volume delves into innovative methods employed in the fields of molecular and cellular biology to detect and study cell death, particularly in the context of cancer and cancer therapy. Spanning various chapters, this book explores essential topics such as non-destructive, real-time Annexin V apoptosis assay, the evaluation of the immune response to tumor cell apoptosis and efferocytosis, the utilization of mass cytometry to assess cell phenotypes and signaling states in human whole blood, the analysis of cell cycle dynamics in response to ER stress and autophagy, and the simultaneous detection of inflammasome activation and membrane damage during pyroptosis. Written in the renowned Methods in Molecular Biology series format, each chapter offers an introduction to its respective topic, a comprehensive list of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable tips on troubleshooting and avoiding known pitfalls.

By presenting cutting-edge and practical approaches, Apoptosis and Cancer: Methods and Protocols serves as a valuable resource and practical guide for both novice and expert researchers seeking to unravel the complexities of cell death. This book empowers researchers to explore the "meaning of death" in cancer and its therapeutic implications, paving the way for advancements in cancer research and treatment.

Weight: 627g
Dimension: 254 x 178 (mm)
ISBN-13: 9781071625521
Edition number: 1st ed. 2022